Insights into the impact of silver nanoparticles on human keratinocytes metabolism through NMR metabolomics

Archives of Biochemistry and Biophysics
Joana CarrolaI F Duarte

Abstract

Due to their antimicrobial properties, silver nanoparticles (AgNPs) are increasingly incorporated into consumer goods and medical products. Their potential toxicity to human cells is however a major concern, and there is a need for improved understanding of their effects on cell metabolism and function. Here, Nuclear Magnetic Resonance (NMR) metabolomics was used to investigate the metabolic profile of human epidermis keratinocytes (HaCaT cell line) exposed for 48 h to 30 nm citrate-stabilized spherical AgNPs (10 and 40 μg/mL). Intracellular aqueous extracts, organic extracts and extracellular culture medium were analysed to provide an integrated view of the cellular metabolic response. The specific metabolite variations, highlighted through multivariate analysis and confirmed by spectral integration, suggested that HaCaT cells exposed to AgNPs displayed upregulated glutathione-based antioxidant protection, increased glutaminolysis, downregulated tricarboxylic acid (TCA) cycle activity, energy depletion and cell membrane modification. Importantly, most metabolic changes were apparent in cells exposed to a concentration of AgNPs which did not affect cell viability at significant levels, thus underlying the sensitivity of NMR met...Continue Reading

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Citations

Nov 14, 2015·Analytical Chemistry·Ian L Gunsolus, Christy L Haynes
Oct 18, 2016·Biomaterials Science·Suhas RameshLuis H Cumbal
Jun 13, 2018·Nanoscale·Javier Frontiñán-RubioEster Vázquez
May 28, 2019·International Journal of Molecular Sciences·Cristina Alexandra Ciocan-CartitaIoana Berindan-Neagoe
Oct 1, 2017·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ivana JarakIola F Duarte
Dec 17, 2019·Colloids and Surfaces. B, Biointerfaces·Mónica CicuéndezIola F Duarte
Jun 9, 2021·Metallomics : Integrated Biometal Science·Yabo LiuZhiyong Zhang
Jan 14, 2020·Chemical Research in Toxicology·Veronica Montesinos-CruzRodrigo Franco

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