Insights into the NrpR regulon in Methanosarcina mazei Gö1

Archives of Microbiology
Katrin WeidenbachRuth A Schmitz

Abstract

The methanogenic archaeon Methanosarcina mazei strain Gö1 contains two homologues of NrpR, the transcriptional repressor of nitrogen assimilation genes recently discovered and characterized in Methanococcus maripaludis. Insertion of a puromycin-resistance conferring cassette into MM1085 encoding a single NrpR domain with an N-terminal helix-turn-helix domain (NrpRI) lead to a significant reduction of the lag-phase after a shift from nitrogen sufficiency to nitrogen limitation. Consistent with this finding, loss of NrpRI resulted in significantly increased transcript levels of genes involved in nitrogen fixation or nitrogen assimilation though growing under nitrogen sufficiency as demonstrated by quantitative reverse transcriptional PCR analysis. Genome-wide analysis using DNA-microarrays confirmed that transcript levels of 27 ORFs were significantly elevated in the M. mazei MM1085::pac mutant under nitrogen sufficiency, including genes known to be up-regulated under nitrogen limitation (e.g., nifH, glnA(1), glnK(1)), and 17 additional genes involved in metabolism (4), encoding a flagella related protein (1) and genes encoding hypothetical proteins (12). Using cell extracts of Escherichia coli expressing MM1085 fused to the malt...Continue Reading

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Citations

Dec 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Dominik JägerRuth A Schmitz
Sep 12, 2012·Nucleic Acids Research·Dominik JägerRuth A Schmitz
May 7, 2013·Applied and Environmental Microbiology·Eoin GunnigleVincent O'Flaherty
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Dec 2, 2017·Molecular Microbiology·Paushali ChaudhurySonja-Verena Albers
Mar 3, 2015·Environmental Microbiology Reports·Zhe Lyu, Yahai Lu
Dec 23, 2017·Molecular Microbiology·Anne BuddewegRuth A Schmitz
Aug 23, 2020·Applied and Environmental Microbiology·Ahmed E Dhamad, Daniel J Lessner

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