Insights of the effects of polyethylene glycol 400 on mammalian and avian nerve terminals

Muscle & Nerve
Mário OshimaYoko Oshima-Franco

Abstract

Polyethylene glycol (PEG) has been widely used as a solvent among other applications. An ideal solvent is one that does not interfere with an in vitro biological system, unless it is a bioactive agent. Herein, a facilitatory neurotransmission effect was exhibited by PEG (20 microM) in mammalian (67 +/- 12.5%, n = 4) and avian (74 +/- 6.8%, n = 6) neuromuscular preparations. In curarized preparations, PEG did not reverse the neurotransmission blockade induced by D-tubocurarine (D-Tc, 5.8 microM, n = 6) as promoted by neostigmine (12 microM, n = 4). A possible presynaptic action of PEG was ruled out, because quantal acetylcholine (ACh) content was similar to the control Tyrode-incubated mammalian preparation. PEG showed improved sarcolemmal sensitivity, both under direct (sarcolemma) and indirect stimulation (motor axon), because it was able to release calcium from the sarcoplasmic reticulum, even when 30 microM dantrolene (n = 5) was previously applied. Neurotransmission decreased at a higher PEG concentration (100 microM, n = -6) in the depolarized membrane, but it did not alter normal muscle fiber morphology. In addition, it partially recovered twitch tension amplitude (55 +/- 5.7%) after washing the preparations. More than a ...Continue Reading

References

Oct 10, 1985·Biochimica Et Biophysica Acta·N Geron, H Meiri
Jan 1, 1972·International Review of Neurobiology·M H Evans
Jun 1, 1965·The Journal of Physiology·D Elmqvist, D M Quastel
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P H O'Lague, S L Huttner
Sep 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·J P GongP Gopalakrishnakone
Feb 28, 2002·Frontiers in Bioscience : a Journal and Virtual Library·Feliciano Protasi
May 17, 2003·Nature Reviews. Drug Discovery·Ruth Duncan
Oct 28, 1955·The Journal of Physiology·A R MARTIN
Sep 1, 1960·British Journal of Pharmacology and Chemotherapy·B L GINSBORG, J WARRINER
Nov 28, 1951·The Journal of Physiology·P FATT, B KATZ
Oct 27, 2004·Basic & Clinical Pharmacology & Toxicology·Yoko Oshima-FrancoLéa Rodrigues-Simioni
Jan 31, 2006·Angewandte Chemie·Rainer Haag, Felix Kratz
Mar 29, 2006·Journal of Pharmacological and Toxicological Methods·Sabine PestelBrian Guth
Jan 20, 2007·Biochemical Society Transactions·D A TomaliaS Svenson
Apr 26, 2007·Orphanet Journal of Rare Diseases·Henry RosenbergKathryn Stowell
Apr 23, 2008·Expert Opinion on Drug Delivery·Sinéad M RyanDavid J Brayden
May 24, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Xiuhua RenGao Li
Jul 22, 2008·Acta Cirúrgica Brasileira·Angélica de Fátima de Assunção BragaLéa Rodrigues Simioni
Sep 27, 2008·PLoS Pathogens·Desigan KumaranSubramanyam Swaminathan
Oct 11, 2008·Journal of Biochemistry·Genghui ZhangHusheng Yan
Nov 21, 2008·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Ralf Gold, Christiane Schneider-Gold
Mar 1, 1946·British Journal of Pharmacology and Chemotherapy·E BULBRING

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Citations

Jun 9, 2012·Toxicon : Official Journal of the International Society on Toxinology·Rita de Cássia O CollaçoPriscila Randazzo-Moura
Dec 4, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Miriéle Cristina FerrazYoko Oshima-Franco

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