Insights on how the activity of an endoglucanase is affected by physical properties of insoluble celluloses

The Journal of Physical Chemistry. B
Juliano BragattoCarlos Driemeier

Abstract

Cellulose physical properties like crystallinity, porosity, and particle size are known to influence cellulase activity, but knowledge is still insufficient for activity prediction from such measurable substrate characteristics. With the aim of illuminating enzyme-substrate relationships, this work evaluates a purified hyperthermophilic endo-1,4-beta-glucanase (from Pyrococcus furiosus) acting on 13 celluloses characterized for crystallinity and crystal width (by X-ray diffraction), wet porosity (by thermoporometry), and particle size (by light scattering). Activities are analyzed by the Michaelis-Menten kinetic equation, which is justified by low enzyme-substrate affinity. Michaelis-Menten coefficients K(m) and k(cat) are reinterpreted in the context of heterogeneous cellulose hydrolysis. For a set of as-received and milled microcrystalline celluloses, activity is successfully described as a function of accessible substrate concentration, with accessibility proportional to K(m)(-1). Accessibility contribution from external particle areas, pore areas, and crystalline packing are discriminated to have comparable magnitudes, implying that activity prediction demands all these substrate properties to be considered. Results additio...Continue Reading

References

May 1, 1978·Biotechnology and Bioengineering·M Okazaki, M Moo-Young
Jan 1, 1994·FEMS Microbiology Reviews·P Béguin, J P Aubert
Oct 9, 1999·Biotechnology Progress·S D MansfieldJ N Saddler
May 4, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·C GrasshoffL Szinicz
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Lee R LyndIsak S Pretorius
Jul 15, 2003·Biomacromolecules·Yoshiharu NishiyamaPaul Langan
Oct 11, 2003·Biotechnology Advances·M K Bhat
Apr 1, 2004·Applied Biochemistry and Biotechnology·Sasithorn KongruangMichael H Penner
Nov 13, 2004·Biotechnology and Bioengineering·Yi-Heng Percival Zhang, Lee R Lynd
May 11, 2006·Biotechnology and Bioengineering·Y-H Percival Zhang, Lee R Lynd
Feb 10, 2007·Science·Michael E HimmelThomas D Foust
Mar 6, 2007·Biotechnology and Bioengineering·Tina JeohDavid K Johnson
Mar 6, 2007·Journal of Agricultural and Food Chemistry·Claudia I IshizawaDavid K Johnson
Feb 9, 2008·Nature Biotechnology·Lee R LyndCharles E Wyman
Mar 5, 1994·Biotechnology and Bioengineering·F M GamaM Mota
Feb 13, 2010·The FEBS Journal·Mélanie HallAndreas S Bommarius
Feb 25, 2010·Biotechnology for Biofuels·Valdeir Arantes, Jack N Saddler
Jun 21, 2011·Applied and Environmental Microbiology·Hongliang WangRolf Prade

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Citations

Jun 7, 2013·Journal of Agricultural and Food Chemistry·Priscila MazieroCarlos Driemeier
Aug 1, 2014·Applied Microbiology and Biotechnology·Rosana GoldbeckFabio M Squina
Dec 22, 2012·The Journal of Physical Chemistry. B·Carlos Driemeier, Juliano Bragatto

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