Insights on the interactions of synthetic amphipathic peptides with model membranes as revealed by 31P and 2H solid-state NMR and infrared spectroscopies

Biophysical Journal
Marise OuelletMichèle Auger

Abstract

We studied the interaction between synthetic amphipathic peptides and model membranes by solid-state NMR and infrared spectroscopies. Peptides with 14 and 21 amino acids composed of leucines and phenylalanines modified by the addition of crown ethers were synthesized. The 14-mer and 21-mer peptides both possess a helical amphipathic structure. To shed light on their membrane interaction, (31)P and (2)H solid-state NMR experiments were performed on both peptides in interaction with dimyristoylphosphatidylcholine vesicles in the absence and presence of cholesterol, dimyristoylphosphatidylglycerol vesicles, and oriented bicelles. (31)P NMR experiments on multilamellar vesicles reveal that the dynamics and/or orientation of the polar headgroups are weakly yet markedly affected by the presence of the peptides, whereas (31)P NMR experiments on bicelles indicate no significant changes in the morphology and orientation of the bicelles. On the other hand, (2)H NMR experiments on vesicles reveal that the acyl chain order is affected differently depending on the membrane lipidic composition and on the peptide hydrophobic length. Finally, infrared spectroscopy was used to study the interfacial region of the bilayer. Based on these studies,...Continue Reading

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D L Worcester
Mar 1, 1991·Chemistry and Physics of Lipids·H H Mantsch, R N McElhaney
Apr 1, 1987·Biophysical Journal·J B SpeyerR G Griffin
Feb 1, 1980·Quarterly Reviews of Biophysics·J Seelig, A Seelig
Apr 8, 1993·Biochimica Et Biophysica Acta·X QiuC Pidgeon
Oct 15, 1996·European Journal of Biochemistry·J Villalaín
Mar 6, 1998·Trends in Biotechnology·R E Hancock, R Lehrer
Jun 23, 1998·Quarterly Reviews of Biophysics·L K Tamm, S A Tatulian
Jan 1, 1999·Chemistry and Physics of Lipids·J L Arrondo, F M Goñi
Jan 29, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·P M Hwang, H J Vogel
Dec 11, 1999·Biochimica Et Biophysica Acta·R M Epand, H J Vogel
Jul 19, 2002·Biophysical Journal·Kevin J HallockA Ramamoorthy
Aug 29, 2002·Chemical Communications : Chem Comm·Yvonne R VandenburgNormand Voyer
Feb 25, 2003·Chemistry and Physics of Lipids·Isabelle MarcotteFrances Separovic

❮ Previous
Next ❯

Citations

May 1, 2013·Accounts of Chemical Research·François OtisNormand Voyer
Sep 29, 2011·Biochemistry·Aurélien LorinMichèle Auger
Aug 26, 2009·Biochimica Et Biophysica Acta·Marise OuelletMichèle Auger
Oct 22, 2019·Biochimica Et Biophysica Acta. Biomembranes·Katarzyna TrzeciakMarek J Potrzebowski
Sep 25, 2020·Biochimica Et Biophysica Acta. Biomembranes·Erick J Dufourc
Jan 21, 2020·Biochimica Et Biophysica Acta. Biomembranes·Miranda L Schmidt, James H Davis
May 3, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Weihua YeLena Mäler
Aug 28, 2012·Chemical Reviews·Ulrich H N DürrAyyalusamy Ramamoorthy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.