Insoluble collagen matrices for prolonged delivery of proteins

Pharmaceutical Development and Technology
W FriessM J Groves

Abstract

The purpose of this investigation was to evaluate the application of high molecular weight, insoluble collagen as a carrier material for proteins. Matrices were formulated and their behavior in buffer solution was investigated with focus on swelling and inner structure. Cross-linking with glutaraldehyde was introduced prior to the formation of the devices and its influence characterized. In addition, the enzymatic degradation process was studied and release experiments with systems loaded with fluorescent-labeled bovine serum albumin were carried out. Insoluble collagen matrices were characterized by intensive swelling in buffer resulting in development of a coarse porous character. Cross-linking strongly reduced the water penetration, leading to denser structures of the swollen devices. The continuous enzymatic degradation of the disk-shaped matrices by collagenase followed the kinetics of an heterogeneous enzymatic process with hindrance of proteolysis by the addition of glutaraldehyde. Release studies demonstrated that large amounts of model protein were held in the matrices with increased cross-linking degree. In presence of collagenase a prolonged release of the trapped protein over several days by matrix cleavage could be...Continue Reading

References

Jan 1, 1992·Biomaterials·T OkadaY Ikada
Jan 1, 1989·Matrix : Collagen and Related Research·J KoppJ P Renou
Nov 1, 1988·Archives of Ophthalmology·R B PhinneyB J Mondino
Jan 1, 1982·Biomaterials·R F OliverR A Grant
Mar 15, 1981·Analytical Biochemistry·I S JamallS S Que Hee
Jan 1, 1995·Journal of Microencapsulation·B RösslerD Scherer
May 1, 1994·Journal of Microencapsulation·C NastruzziE Menegatti
Dec 1, 1996·Biomaterials·W Friess, G Lee

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Citations

Apr 12, 2002·Journal of Pharmaceutical Sciences·Florin A RaduWolfgang C Friess
Sep 16, 2015·Journal of Oral Science·Xiaoqiang LiuJianguo Tan
Nov 28, 2012·Journal of Biomedical Materials Research. Part a·Umer DaoodAmr S Fawzy
Jul 19, 2002·Biophysical Journal·Abraham R TzafririHanna Parnas
Dec 12, 2012·Scientific Reports·Goichi MatsumotoHajime Mori
Aug 15, 1998·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·W Friess

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