PMID: 6964393Oct 1, 1982Paper

Instability of coated vesicles in concentrated sucrose solutions

Proceedings of the National Academy of Sciences of the United States of America
P K NandiH Edelhoch

Abstract

A method of preparing homogeneous coated vesicles that eliminates the high sucrose concentrations heretofore used is presented. It is shown that sucrose at high concentrations dissociates the coat from coated vesicles. This reaction can explain the presence of empty coats observed with preparations obtained with high concentrations of sucrose. The protein and membrane lipid components have been analyzed by the intrinsic tryptophan and extrinsic diphenylhexatriene fluorescence, respectively. Analysis of mixtures of coated vesicles and baskets resolved the contributions of the two species to the fluorescence curves.

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M P Woodward, T F Roth
Dec 25, 1978·Journal of Molecular Biology·B M Pearse
Jun 21, 1979·Nature·J L GoldsteinM S Brown
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M S Brown, J L Goldstein
Jun 5, 1976·Journal of Molecular Biology·R A CrowtherB M Pearse
Sep 5, 1975·Journal of Molecular Biology·B M Pearse
Apr 1, 1976·The Journal of Cell Biology·W W FrankeT W Keenan
Oct 9, 1973·Biochemistry·A Zipp, W Kauzmann
Nov 1, 1967·The Journal of Cell Biology·D S Friend, M G Farquhar
May 1, 1981·The Journal of Cell Biology·J L RubensteinJ E Rothman
Mar 1, 1980·The Journal of Cell Biology·J Heuser
Oct 1, 1981·The Journal of Cell Biology·J L CarpentierL Orci
Feb 1, 1964·The Journal of Cell Biology·T F ROTH, K R PORTER

❮ Previous
Next ❯

Citations

Jan 1, 1992·Cell Motility and the Cytoskeleton·P L LeopoldS T Brady
Dec 1, 1995·Molecular Reproduction and Development·A V VieiraP M Vieira
Jun 1, 1993·Cell and Tissue Research·X ShenW J Schneider
Mar 1, 1985·Planta·B G MerseyL C Fowke
Sep 18, 1987·Biochimica Et Biophysica Acta·R LawaczeckC Nicolau
Sep 8, 1993·Biochimica Et Biophysica Acta·A V Vieira, W J Schneider
Jan 1, 1985·Neurochemistry International·H R Knull
Jun 29, 1984·Biochimica Et Biophysica Acta·A PauloinP Jollés
Jul 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·T YoraH Nakamura
Feb 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T YoraH Nakamura
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R W Van DykeB F Scharschmidt
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·W J Schook, S Puszkin
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·X S XieD K Stone
May 1, 1986·The Journal of Cell Biology·J Lippincott-Schwartz, D M Fambrough
Jul 1, 1986·The Journal of Cell Biology·N L KedershaL H Rome
May 1, 1989·The Journal of Cell Biology·D T MahaffeyR G Anderson
Oct 1, 1983·The Journal of Cell Biology·W G KellyT F Roth
May 3, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jennifer R MorganEileen M Lafer
Jan 1, 1984·European Biophysics Journal : EBJ·A AlfsenH Edelhoch
Nov 5, 2008·The Journal of Cell Biology·Sophia SemerdjievaElizabeth Smythe
Feb 14, 2006·Biophysical Journal·Albert J JinRalph Nossal
Oct 15, 1983·Archives of Biochemistry and Biophysics·R NossalH Edelhoch
Dec 1, 1984·Archives of Biochemistry and Biophysics·K PrasadH Edelhoch
Apr 13, 1990·Biochimica Et Biophysica Acta·J P PrivatM Ptak
Aug 15, 1985·Archives of Biochemistry and Biophysics·R ZarrilliP K Nandi
Sep 1, 1989·Archives of Biochemistry and Biophysics·F P SchwarzW H Kirchhoff
May 22, 2013·Journal of Structural Biology·J Bernard HeymannAlasdair C Steven
Dec 7, 1982·Biochemistry·P K NandiH Edelhoch

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.