Instability of plastid DNA in the nuclear genome.

PLoS Genetics
Anna E Sheppard, Jeremy N Timmis

Abstract

Functional gene transfer from the plastid (chloroplast) and mitochondrial genomes to the nucleus has been an important driving force in eukaryotic evolution. Non-functional DNA transfer is far more frequent, and the frequency of such transfers from the plastid to the nucleus has been determined experimentally in tobacco using transplastomic lines containing, in their plastid genome, a kanamycin resistance gene (neo) readymade for nuclear expression. Contrary to expectations, non-Mendelian segregation of the kanamycin resistance phenotype is seen in progeny of some lines in which neo has been transferred to the nuclear genome. Here, we provide a detailed analysis of the instability of kanamycin resistance in nine of these lines, and we show that it is due to deletion of neo. Four lines showed instability with variation between progeny derived from different areas of the same plant, suggesting a loss of neo during somatic cell division. One line showed a consistent reduction in the proportion of kanamycin-resistant progeny, suggesting a loss of neo during meiosis, and the remaining four lines were relatively stable. To avoid genomic enlargement, the high frequency of plastid DNA integration into the nuclear genome necessitates a ...Continue Reading

References

Jun 25, 1992·Nucleic Acids Research·L M MonteroJ Salinas
Sep 1, 1995·The Plant Journal : for Cell and Molecular Biology·Y G LiuR F Whittier
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·F Baudat, A Nicolas
Jun 6, 1998·Molecular Biology and Evolution·M A AyliffeJ N Timmis
Mar 5, 1999·Science·M W GrayB F Lang
Mar 6, 1999·Current Opinion in Plant Biology·A J Matzke, M A Matzke
Jan 5, 2000·Molecular Cell·X Yu, A Gabriel
Feb 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·A Limanton-GrevetM Jullien
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Keith L AdamsJeffrey D Palmer
Sep 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·William MartinDavid Penny
Dec 7, 2002·The Plant Cell·Allen SessionsStephen A Goff
Jun 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sandra StegemannRalph Bock
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Chun Y HuangJeremy N Timmis
Jul 16, 2004·Molecular Biology and Evolution·Erik Richly, Dario Leister
Jul 30, 2005·Current Biology : CB·Naiara Rodríguez-EzpeletaB Franz Lang
Dec 5, 2006·Current Biology : CB·Adrian Reyes-PrietoDebashish Bhattacharya
Nov 6, 2007·Trends in Genetics : TIG·Christos NoutsosDario Leister
Jul 29, 2008·Plant Physiology·Anna E SheppardJeremy N Timmis
Jun 1, 1996·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·V SrivastavaI K Vasil
Nov 1, 1992·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M A Ayliffe, J N Timmis
Jun 1, 1986·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·T A Kavanagh, J N Timmis

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Citations

Aug 6, 2013·Cellular and Molecular Life Sciences : CMLS·Andreas HoubenJeremy N Timmis
Oct 31, 2013·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Takanori YoshidaAkira Kawabe
Aug 3, 2014·Genome Biology and Evolution·Chih-Yao HsuShu-Miaw Chaw
Feb 20, 2010·PLoS Genetics·Einat Hazkani-CovoWilliam Martin
Mar 23, 2011·Plant Molecular Biology·Susann WickeDietmar Quandt
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Dong WangJeremy N Timmis
Mar 14, 2012·PloS One·Hubert H Rogers, Sam Griffiths-Jones
Jan 22, 2011·Molecular Biology and Evolution·Andrew H Lloyd, Jeremy N Timmis
Mar 16, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Sungyul ChangLeslie L Domier
May 11, 2013·Genome Biology and Evolution·Dong Wang, Jeremy N Timmis
Jan 12, 2016·Scientific Reports·Briardo LlorenteManuel Rodríguez-Concepción
Jan 31, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Stephan Greiner, Ralph Bock
Aug 5, 2015·BMC Genomics·Libia Catalina Salinas CastellanosJorge Hernández-Torres
Apr 6, 2012·Mobile Genetic Elements·Andrew H Lloyd, Jeremy N Timmis
Aug 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Mihaela Maria MartisAndreas Houben
Apr 22, 2017·Genome Biology and Evolution·Xiao-Jian QuTing-Shuang Yi
Apr 27, 2017·Genome Biology and Evolution·Einat Hazkani-Covo, William F Martin
Jul 19, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jiantao ZhaoJing Zhang
Oct 27, 2018·Genes·André MarquesAndreas Houben
Nov 21, 2017·The Plant Journal : for Cell and Molecular Biology·Dong WangJeremy N Timmis
May 13, 2020·Genome Génome / Conseil National De Recherches Canada·María J Puertas, Mónica González-Sánchez
Nov 14, 2019·BMC Plant Biology·Nan ZhaoJinping Hua
Sep 4, 2015·G3 : Genes - Genomes - Genetics·Ashley N LoughKathleen J Newton
Jan 25, 2020·International Journal of Molecular Sciences·Guo-Jun ZhangHong-Xing Niu

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