Installing extra bicarbonate transporters in the cyanobacterium Synechocystis sp. PCC6803 enhances biomass production

Metabolic Engineering
Nina A KamennayaChrister Jansson

Abstract

As a means to improve carbon uptake in the cyanobacterium Synechocystis sp. strain PCC6803, we engineered strains to contain additional inducible copies of the endogenous bicarbonate transporter BicA, an essential component of the CO2-concentrating mechanism in cyanobacteria. When cultured under atmospheric CO2 pressure, the strain expressing extra BicA transporters (BicA(+) strain) grew almost twice as fast and accumulated almost twice as much biomass as the control strain. When enriched with 0.5% or 5% CO2, the BicA(+) strain grew slower than the control but still showed a superior biomass production. Introducing a point mutation in the large C-terminal cytosolic domain of the inserted BicA, at a site implicated in allosteric regulation of transport activity, resulted in a strain (BicA(+)(T485G) strain) that exhibited pronounced cell aggregation and failed to grow at 5% CO2. However, the bicarbonate uptake capacity of the induced BicA(+)(T485G) was twice higher than for the wild-type strain. Metabolic analyses, including phenotyping by synchrotron-radiation Fourier transform Infrared spectromicroscopy, scanning electron microscopy, and lectin staining, suggest that the excess assimilated carbon in BicA(+) and BicA(+)(T485G) c...Continue Reading

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Citations

Feb 18, 2016·Applied Microbiology and Biotechnology·Xinyan GaoWeiwen Zhang
Jan 9, 2016·Microbial Cell Factories·Jie ZhouYin Li
Apr 12, 2016·Frontiers in Microbiology·Chiranjib BanerjeePratyoosh Shukla
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Nov 5, 2019·Geobiology·Nina A KamennayaChrister Jansson
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Mar 16, 2019·Frontiers in Bioengineering and Biotechnology·María Santos-MerinoDaniel C Ducat
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Aug 20, 2021·Current Opinion in Biotechnology·Xufeng LiuPeter Lindblad
Jan 8, 2021·Journal of Agricultural and Food Chemistry·Hyun Jeong LeeHan Min Woo

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