Instant intra-operative neutropenia despite the emergence of banded (CD16dim/CD62Lbright) neutrophils in peripheral blood - An observational study during extensive trauma-surgery in pigs.

Injury
Michel TeubenLuke P H Leenen

Abstract

Deregulation of polymorphonuclear neutrophils (PMNs) is an essential step in the development of inflammatory complications upon trauma. Different neutrophil subtypes have been identified recently, however, the role of neutrophil subtypes in immunoregulation upon trauma is unclear. We hypothesize that extensive trauma surgery causes instant progressive heterogeneity of the blood neutrophil pool, and increased appearance of young (CD16dim/CD62Lbright) neutrophils in peripheral blood. A standardized extensive thoraco-abdominal porcine trauma surgery model was utilized, and 12 animals were included. Blood was collected at defined timepoints and neutrophil numbers and subtypes were studied by flowcytometry. Neutrophil subtypes were identified by differences in cell surface expression levels of CD16 (FcγRIII) and CD62L (L-selectin). Porcine neutrophil subtypes were further characterized after flow sorting. Eleven animals survived the 3-hour surgical protocol. Neutrophil numbers dropped significantly from a mean of 8,6 ± 3,5 × 106 to 2,4 ± 1,8 × 106 cells/ml during 180 min, (p<0.001). Simultaneously, the blood PMN population became increasingly heterogeneous due to the appearance of new neutrophil subtypes. Cell sorting experiments an...Continue Reading

References

Feb 1, 1989·Annals of Surgery·I S PatersonH B Hechtman
Jan 1, 1981·The Journal of Pediatrics·R D ChristensenG Rothstein
Jan 1, 1993·The British Journal of Surgery·A C WindsorH J Sugerman
Mar 13, 2002·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Gulbu IsitmangilErdogan Kunter
Mar 6, 2004·Science·Volker BrinkmannArturo Zychlinsky
May 25, 2005·Injury·Marius Keel, Otmar Trentz
Jul 11, 2006·Lancet·K A BrownD F Treacher
Apr 17, 2009·Nature Reviews. Microbiology·Ronald S FlannaganSergio Grinstein
Jul 14, 2010·Trends in Immunology·Charlotte SummersEdwin R Chilvers
Apr 16, 2011·Thrombosis Research·Ulf KesslerStephan M Jakob
Dec 14, 2011·The Journal of Clinical Investigation·Janesh PillayLeo Koenderman
Aug 29, 2012·Journal of Leukocyte Biology·Vera M KampLeo Koenderman
Jul 17, 2013·Shock·Frank HildebrandMartijn van Griensven
Aug 27, 2015·The Journal of Trauma and Acute Care Surgery·Brian C GulackMark L Shapiro
Apr 14, 2016·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Delphine SauceCarole Elbim
Mar 9, 2018·Frontiers in Physiology·Carlos Rosales
Jun 14, 2018·Blood Advances·Pieter H C LeliefeldLeo Koenderman
Jun 6, 2019·World Journal of Emergency Surgery : WJES·Lillian HesselinkFalco Hietbrink
Oct 16, 2019·European Journal of Trauma and Emergency Surgery : Official Publication of the European Trauma Society·Borna Relja, Walter Gottlieb Land
Oct 17, 2019·Frontiers in Immunology·Pacôme LecotMarie-Cécile Michallet

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.