Instant transport media for biopsied soft tissue specimens: a comparative study

Scientifica
Shankargouda PatilA Thirumal Raj

Abstract

Background. Formalin, a traditionally preferred fixative in the field of pathology, has restricted usage in private settings. Since its toxicity violates the Occupational Safety and Health Administration regulations, an eco-friendly alternative would be the need of the hour. Hence an instant media which is economical and nontoxic and enables easy transport of biopsied soft tissue specimens in its original state is of vital importance. Materials and Methods. Commercially available fresh goat buccal mucosa specimens were sliced into smaller bits of equal dimensions and placed in six different containers containing 20% honey, 30% jaggery, milk, and ice for 1 hr, 6 hours, 12 hours, and 24 hours each with formalin as a positive control. After the set time interval, the specimens were fixed in 10% buffered formalin for 24 hours followed by routine processing and staining. The histologic interpretations were a blinded procedure and evaluated by two experts. Results were statistically analysed. Results. 30% jaggery proved to be an ideal transport media showing high quality preservation after 24 hours. 20% honey and ice showed optimal tissue preservation up to 6 hours following which quality deteriorated. Tissues transported in milk sho...Continue Reading

References

Aug 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C H FoxP P Roller
Nov 11, 2008·Annals of Diagnostic Pathology·René J Buesa
Feb 24, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Kun LuJames A Swenberg
Aug 24, 2011·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·N OzkanB Tüzüner

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