Instant Typing Is Essential to Detect Transmission of Extended-Spectrum Beta-Lactamase-Producing Klebsiella Species

PloS One
Anne F Voor In 't HoltMargreet C Vos

Abstract

Infections with multidrug-resistant (MDR) microorganisms are an increasing threat to hospitalized patients. Although rapid typing of MDR microorganisms is required to apply targeted prevention measures, technical barriers often prevent this. We aimed to assess whether extended-spectrum beta-lactamase (ESBL)-producing Klebsiella species are transmitted between patients and whether routine, rapid typing is needed. For 43 months, the clonality of all ESBL-producing Klebsiella isolates from patients admitted to Erasmus MC University Medical Center in Rotterdam, the Netherlands was assessed with Raman spectroscopy. A cluster was defined as n ≥ 2 patients who had identical isolates. Primary patients were the first patients in each cluster. Secondary patients were those identified with an isolate clonally related to the isolate of the primary patient. Isolates from 132 patients were analyzed. We identified 17 clusters, with 17 primary and 56 secondary patients. Fifty-nine patients had a unique isolate. Patients (n = 15) in four out of the 17 clusters were epidemiologically related. Ten of these 15 patients developed an infection. Clonal outbreaks of ESBL-producing Klebsiella species were detected in our hospital. Theoretically, after ...Continue Reading

References

Feb 1, 1985·Infection Control : IC·W R JarvisJ M Hughes
Feb 14, 2004·The Journal of Antimicrobial Chemotherapy·Nicola A C PotzDavid M Livermore
Jun 7, 2005·Journal of Microbiological Methods·Kees MaquelinGerwin J Puppels
Aug 18, 2006·BMC Infectious Diseases·Axel KramerGünter Kampf
Oct 31, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A D HarrisJ A Johnson
Dec 26, 2008·Journal of Clinical Microbiology·Diana F M Willemse-ErixKees Maquelin
Jan 2, 2009·The New England Journal of Medicine·A M G A de SmetM J M Bonten
Aug 19, 2009·The Journal of Antimicrobial Chemotherapy·Peter M Hawkey, Annie M Jones
Aug 22, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A MichalopoulosM E Falagas
Aug 25, 2010·Antimicrobial Agents and Chemotherapy·Mical PaulLeonard Leibovici
Sep 23, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Y ChongT Kamimura
Jan 18, 2012·Tropical Medicine & International Health : TM & IH·Juliëtte A SeverinWil H Goessens
Jun 19, 2012·The Korean Journal of Internal Medicine·David M Livermore
Mar 15, 2014·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·M J DautzenbergM J Bonten

❮ Previous
Next ❯

Citations

Mar 24, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Aryann R GanjoWil Goessens
Aug 12, 2016·Journal of Clinical Microbiology·Jolene R BowersPaul Keim
Nov 3, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Céline SlekovecUNKNOWN DPCPYO Trial Group

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

SpectraCell RA RA
Excel

Related Concepts

Related Feeds

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.