Nov 1, 2018

Instantaneous isotropic volumetric imaging of fast biological processes

BioRxiv : the Preprint Server for Biology
Nils WagnerRobert Prevedel

Abstract

Capturing highly dynamic biological processes at sub-cellular resolution is a recurring challenge in biology. Here we show that combining selective volume illumination with simultaneous acquisition of orthogonal light-fields yields 3D images with high, isotropic spatial resolution and free of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate Medaka heart and blood flow imaging with single-cell resolution and free of motion artefacts at volume rates up to 200Hz.

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Mentioned in this Paper

Reconstructive Surgical Procedures
Spatial Distribution
Blood Flow
Microscopy
Heart
Illumination Technique
Illumination (Procedure)
Cell Separation
Diagnostic Imaging
Oryzias latipes

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