Instantaneous pH-Boosted Functionalization of Stellate Gold Nanoparticles for Intracellular Imaging of miRNA

ACS Applied Materials & Interfaces
Jisun KiSeungjoo Haam

Abstract

Various types of nanoprobes have recently been utilized to monitor living organisms by detecting and imaging intracellular biomarkers, such as microRNAs (miRs). We here present a simple one-pot method to prepare stellate gold nanoparticles functionalized with miR-detecting molecular beacons (SGNP-MBs); low pH conditions permitted the rapid-high loading of MBs on the surface of SGNPs. Compared to the conventional gold nanoparticle-based MBs, SGNPs carried a 4.5-fold higher load of MBs and exhibited a 6.4-fold higher cellular uptake. We demonstrated that SGNP-MBs were successfully internalized in human gastric cancer cell lines and could be used to accurately detect and image intracellular miRs in an miR-specific manner. Furthermore, the relative levels of intracellular miRs in three different cell lines expressing miR-10b (high, moderate, and low levels) could be monitored using SGNP-MBs. Consequently, these results indicated that SGNP-MBs could have applications as highly potent, efficient nanoprobes to assess intracellular miR levels in living cells.

References

Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·D L SokolA M Gewirtz
Feb 6, 2003·Journal of the American Chemical Society·Rongchao JinGeorge C Schatz
Feb 13, 2003·Nucleic Acids Research·Andrew TsourkasGang Bao
Apr 30, 2003·Biophysical Journal·Daniel P AalbertsNoel L Goddard
Sep 15, 2005·Journal of the American Chemical Society·Abigail K R Lytton-Jean, Chad A Mirkin
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefano VoliniaCarlo M Croce
Apr 13, 2006·Nano Letters·B Devika ChithraniWarren C W Chan
May 20, 2006·Science·Nathaniel L RosiChad A Mirkin
Oct 25, 2006·Nature Reviews. Cancer·George A Calin, Carlo M Croce
Jun 17, 2008·Advanced Drug Delivery Reviews·Partha GhoshVincent M Rotello
Aug 26, 2009·ACS Nano·Andrew E PrigodichChad A Mirkin
Nov 12, 2010·Breast Cancer Research : BCR·Li Ma
Jul 19, 2011·Nature Nanotechnology·Weian ZhaoJeffrey M Karp
Nov 24, 2011·Nano Reviews·Panikkanvalappil R SajanlalThalappil Pradeep
Jun 16, 2012·Biomaterials·Won Jun KangSoonhag Kim
Sep 7, 2012·Chemical Communications : Chem Comm·Xu ZhangJuewen Liu
Jan 29, 2013·Human Pathology·Yuan-Yu WangXu-Jun He
Feb 21, 2013·Chemical Communications : Chem Comm·Dangwei ShiBaoquan Ding
Feb 28, 2013·Metallomics : Integrated Biometal Science·Germán Plascencia-VillaMiguel José-Yacamán
Sep 3, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hailin TangXiaoming Xie
Dec 20, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Fengju SongKexin Chen
Jan 8, 2014·Chemical Society Reviews·Hui XuChunhai Fan
Dec 3, 2014·Nanomedicine : Nanotechnology, Biology, and Medicine·Duncan Hieu M DamTeri W Odom
May 23, 2015·Journal of the American Chemical Society·Chun Kit K ChoiLiming Bian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved