Instructive role of M-CSF on commitment of bipotent myeloid cells involves ERK-dependent positive and negative signaling

Journal of Leukocyte Biology
Sylvain CarrasStéphanie Gobert

Abstract

M-CSF and G-CSF are instructive cytokines that specifically induce differentiation of bipotent myeloid progenitors into macrophages and granulocytes, respectively. Through morphology and colony assay studies, flow cytometry analysis of specific markers, and expression of myeloid transcription factors, we show here that the Eger/Fms cell line is composed of cells whose differentiation fate is instructed by M-CSF and G-CSF, thus representing a good in vitro model of myeloid bipotent progenitors. Consistent with the essential role of ERK1/2 during macrophage differentiation and defects of macrophagic differentiation in native ERK1(-/-) progenitors, ERK signaling is strongly activated in Eger/Fms cells upon M-CSF-induced macrophagic differentiation but only to a very small extent during G-CSF-induced granulocytic differentiation. Previous in vivo studies indicated a key role of Fli-1 in myeloid differentiation and demonstrated its weak expression during macrophagic differentiation with a strong expression during granulocytic differentiation. Here, we demonstrated that this effect could be mediated by a differential regulation of protein kinase Cδ (PKCd) on Fli-1 expression in response to M-CSF and G-CSF. With the use of knockdown o...Continue Reading

References

Aug 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Markus G ManzIrving L Weissman
Nov 24, 2004·Pediatric Research·Patricia Mora-GarciaKathleen M Sakamoto
Apr 20, 2007·Leukemia·M B Miranda, D E Johnson
Jul 11, 2009·Science·Michael A RiegerTimm Schroeder
Sep 24, 2010·Leukemia·L T van der MeerB A van der Reijden
Nov 5, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Saminathan MuthusamyJaleh Malakooti
Feb 4, 2012·PloS One·Nathalie SaulnierMurielle Gaudry
Sep 17, 2013·Journal of Cellular Biochemistry·Christiano Marcello Vaz BarbosaEdgar J Paredes-Gamero

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Citations

Feb 6, 2017·Blood·Max EndeleTimm Schroeder

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