Instrument and method to determine the electrophoretic mobility of nanoparticles and proteins by combining electrical and flow field-flow fractionation

Analytical Chemistry
Christoph JohannUlrich Rösch

Abstract

A new FFF method is presented which combines asymmetrical flow-FFF (AF4) and electrical FFF (ElFFF) in one channel to electrical asymmetrical flow-FFF (EAF4) to overcome the restrictions of pure ElFFF. It allows for measuring electrophoretic mobility (μ) as a function of size. The method provides an absolute value and does not require calibration. Results of μ for two particle standards are in good agreement with values determined by phase analysis light scattering (PALS). There is no requirement for low ionic strength carriers with EAF4. This overcomes one of the main limitations of ElFFF, making it feasible to measure proteins under physiological conditions. EAF4 has the capability to determine μ for individual populations which are resolved into separate peaks. This is demonstrated for a mixture of three polystyrene latex particles with different sizes as well as for the monomer and dimer of BSA and an antibody. The experimental setup consists of an AF4 channel with added electrodes; one is placed beneath the frit at the bottom wall and the other covers the inside of the upper channel plate. This design minimizes contamination from the electrolysis reactions by keeping the particles distant from the electrodes. In addition t...Continue Reading

References

Apr 21, 1972·Science·K D CaldwellJ C Giddings
Apr 28, 2000·Analytical Chemistry·N TriR Beckett
Apr 1, 2005·Electrophoresis·Bruce K Gale, Merugu Srinivas
Jul 22, 2011·Analytical Chemistry·Julien GigaultGaëtane Lespes

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Citations

Nov 14, 2015·Analytical Chemistry·Ian L Gunsolus, Christy L Haynes
Jun 24, 2016·Trends in Analytical Chemistry : TRAC·Leena Pitkänen, André M Striegel
Jan 25, 2017·Analytical and Bioanalytical Chemistry·Catia Contado
Feb 2, 2021·Drug Delivery and Translational Research·Federico QuattriniMaría José Alonso
Mar 27, 2021·Annual Review of Analytical Chemistry·Christine L PlavchakS Kim Ratanathanawongs Williams
Sep 15, 2021·Journal of Separation Science·Gaëtane Lespes, Valentin De Carsalade Du Pont
Nov 2, 2021·ACS Nano·Sara Hassanpour TamrinArindom Sen

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