Insulator function and topological domain border strength scale with architectural protein occupancy

Genome Biology
Kevin Van BortleVictor G Corces


Chromosome conformation capture studies suggest that eukaryotic genomes are organized into structures called topologically associating domains. The borders of these domains are highly enriched for architectural proteins with characterized roles in insulator function. However, a majority of architectural protein binding sites localize within topological domains, suggesting sites associated with domain borders represent a functionally different subclass of these regulatory elements. How topologically associating domains are established and what differentiates border-associated from non-border architectural protein binding sites remain unanswered questions. By mapping the genome-wide target sites for several Drosophila architectural proteins, including previously uncharacterized profiles for TFIIIC and SMC-containing condensin complexes, we uncover an extensive pattern of colocalization in which architectural proteins establish dense clusters at the borders of topological domains. Reporter-based enhancer-blocking insulator activity as well as endogenous domain border strength scale with the occupancy level of architectural protein binding sites, suggesting co-binding by architectural proteins underlies the functional potential of ...Continue Reading


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Oct 22, 2014·The Journal of Cell Biology·Chloe E SniderKerry Bloom
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Feb 11, 2016·Trends in Genetics : TIG·Darío G LupiáñezStefan Mundlos
Jan 24, 2016·Current Opinion in Genetics & Development·Tamer AliMarek Bartkuhn
Feb 8, 2016·Current Opinion in Cell Biology·M Jordan Rowley, Victor G Corces
May 27, 2015·FEBS Letters·Caelin Cubeñas-Potts, Victor G Corces
Oct 28, 2015·Proceedings of the National Academy of Sciences of the United States of America·Pierre J FabreDenis Duboule
Sep 30, 2015·Nucleus·Caelin Cubeñas-Potts, Victor G Corces
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Oct 6, 2015·Annual Review of Genetics·Huy Q Nguyen, Giovanni Bosco
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May 15, 2015·Current Opinion in Genetics & Development·Alyssa C Lau, Györgyi Csankovszki
Mar 18, 2015·Nucleus·Antoine Le GallMarcelo Nollmann
Aug 11, 2015·Genome Biology·Britta A M Bouwman, Wouter de Laat
Jun 21, 2015·Current Opinion in Cell Biology·Yuri Frosi, Christian H Haering
Jun 26, 2015·Epigenomics·Pedro P RochaJane A Skok
Dec 3, 2014·Methods : a Companion to Methods in Enzymology·Bryan R LajoieNoam Kaplan
Mar 12, 2015·Nature Reviews. Molecular Cell Biology·Ana Pombo, Niall Dillon
Jan 30, 2015·Frontiers in Genetics·Alyssa C Lau, Györgyi Csankovszki
May 7, 2016·Bioinformatics·Jie ChenIndika Rajapakse
May 24, 2016·Cell Reports·Geoffrey FudenbergLeonid A Mirny
May 26, 2016·Seminars in Cell & Developmental Biology·Krysta L EngelDaniel Savic
Jun 4, 2016·Molecular Cell·Jesse R DixonBing Ren
Jun 30, 2016·Human Molecular Genetics·Malte Spielmann, Stefan Mundlos
Oct 16, 2016·Nature Reviews. Genetics·Boyan Bonev, Giacomo Cavalli
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Kenneth J EvansJulie Ahringer
Jan 7, 2017·Nucleus·A Rasim BarutcuAnthony N Imbalzano
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