PMID: 2122450Oct 1, 1990Paper

Insulin activates a 70-kDa S6 kinase through serine/threonine-specific phosphorylation of the enzyme polypeptide

Proceedings of the National Academy of Sciences of the United States of America
D J PriceJ Avruch

Abstract

The dominant insulin-stimulated ribosomal protein S6 kinase activity was purified to near homogeneity from insulin-treated 32P-labeled rat H4 hepatoma cells and found to copurify with a 70-kDa 32P-labeled polypeptide. The dominant S6 kinase purified from livers of cycloheximide-treated rats is also a 70-kDa polypeptide. Antiserum raised against rat liver S6 kinase specifically immunoprecipitates the purified 32P-labeled H4 hepatoma insulin-stimulated S6 kinase. This antiserum also specifically precipitates insulin-stimulated S6 kinase activity directly from cytosolic extracts of H4 cells. Immune complexes prepared from the cytosol of 32P-labeled H4 cells contain several 32P-labeled polypeptides; only a 70-kDA 32P-labeled peptide, however, is specifically displaced by preadsorption of the antiserum with nonradioactive rat liver S6 kinase. Insulin treatment increases the 32P content of the immunoprecipitated 70-kDa S6 kinase polypeptide 3- to 4-fold over basal levels; 32P-labeled serine, some 32P-labeled threonine, but no 32P-labeled tyrosine are detected after partial acid hydrolysis. Tryptic peptide maps indicate that the insulin-stimulated S6 kinase purified from 32P-labeled H4 cells is phosphorylated at multiple sites distinc...Continue Reading

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Citations

Aug 3, 1994·Biochemical Pharmacology·W K AgbotounouJ Pierre
Oct 1, 1993·Baillière's Clinical Endocrinology and Metabolism·W T Garvey, M J Birnbaum
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P BanerjeeJ Avruch
Dec 1, 1991·Cell Regulation·M H CobbD J Robbins
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·Q P WengJ Avruch
Jun 1, 1995·European Journal of Biochemistry·C PetritschL M Ballou
Mar 10, 1995·The Journal of Biological Chemistry·E Giasson, S Meloche
Jan 19, 2002·The Journal of Biological Chemistry·Terence P HerbertChristopher G Proud
Jul 4, 2003·The Journal of Biological Chemistry·Christopher BelhamJoseph Avruch
Jan 6, 2021·Nucleic Acids Research·Jian-Jun JiaBruno D Fonseca

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