Insulin acts as a somatomedin analog in stimulating myoblast growth in serum-free medium

In Vitro
J R Florini, D Z Ewton

Abstract

A serum-free medium that supports the proliferation of myoblasts (but not of fibroblasts) has been developed recently in this laboratory. It is composed of 10(-6) M insulin, 10(-7) M dexamethasone, and 10(-5) M fetuin, and is designated medium MM-1. The latter two components gave optimal stimulation at or near "physiological" concentrations, but insulin was required at levels far in excess of those found in serum. Accordingly, we have now investigated the possibility that insulin acts as a weak analog of the somatomedins, as has been suggested in other systems. We found that maximal growth rates were observed when 10(-6) M insulin was replaced by 0.5 to 1.0 microgram/ml multiplication stimulating activity (MSA), indicating that insulin serves a somatomedinlike function of MM-1. We also investigated the possibility that a contaminant of fetuin is responsible for its action in MM-1 but found no evidence to support this suggestion. We conclude that MM-1 is suitable for the study of muscle cell growth and differentiation under rather well-defined conditions, and that insulin probably is serving as a somatomedin analog in this medium.

References

Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A Rizzino, G Sato
Dec 1, 1979·In Vitro·J R Florini, S B Roberts
Feb 19, 1975·Biochimica Et Biophysica Acta·W C DuckworthA E Kitabchi
Sep 1, 1979·Experimental Cell Research·T R PodleskiK M Yamada
Aug 1, 1979·Journal of Cellular Physiology·G F MerrillJ R Florini
Jan 1, 1978·In Vitro·I HayashiG Sato
Jan 1, 1976·Annual Review of Biochemistry·D Gospodarowicz, J S Moran
Jul 1, 1977·Endocrinology·J R FloriniN C Dulak
Feb 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·K NishikawaG Sato
Oct 18, 1974·Nature·J L Mandel, M L Pearson
Apr 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·L S JeffersonH E Morgan
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·D Yaffe
Aug 1, 1981·Developmental Biology·D Z Ewton, J R Florini
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A Rizzino, C Crowley
Jan 1, 1980·Journal of Cellular Physiology·E H Ball, B D Sanwal
Jan 1, 1980·Journal of Supramolecular Structure·T A LinkhartS D Hauschka
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A C MosesO Z Higa
Sep 10, 1962·Biochimica Et Biophysica Acta·P R DAVOREN
Jan 1, 1958·Proceedings of the National Academy of Sciences of the United States of America·H W FisherG Sato
Dec 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·G de L HabaV Elting

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Citations

Feb 15, 1993·Journal of Neuroscience Research·J M LylesC L Weill
Dec 1, 1984·In Vitro·J R FloriniB M Vertel
Nov 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·R E AllenL K Boxhorn
Jul 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D Gospodarowicz, J Cheng
Mar 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·I KimuraE Ozawa
Apr 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·L E Shapiro, N Wagner
Jan 1, 1984·Neuroscience and Biobehavioral Reviews·H J RomijnP S Wolters
Jan 1, 1984·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·E YolesS R Sampson
Jan 1, 1986·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·C Brodie, S R Sampson
Jan 1, 1992·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·J M LylesC L Weill
May 4, 2000·Domestic Animal Endocrinology·C L ReichelD E Gerrard
Sep 1, 1987·Muscle & Nerve·J R Florini
Sep 1, 1984·Journal of Cellular Physiology·D Z EwtonJ R Florini
Oct 22, 2011·Neurobiology of Aging·Doris P MolinaMichelle M Adams
Mar 1, 1984·Clinics in Endocrinology and Metabolism
Apr 6, 2000·American Journal of Physiology. Endocrinology and Metabolism·M Fournier, M I Lewis
Nov 1, 1986·The American Journal of Physiology·M ShimizuR A Roth
Apr 1, 1989·The American Journal of Physiology·J R Florini, K A Magri
Sep 1, 1992·The American Journal of Physiology·Z Nie
Mar 1, 1984·Clinics in Endocrinology and Metabolism·S P Nissley, M M Rechler

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