Jul 21, 1976

Insulin and glucagon degradation by the kidney. I. Subcellular distribution under different assay condition

Biochimica Et Biophysica Acta
W C Duckworth

Abstract

Insulin and glucagon degradation by rat kidney homogenates and subcellular fractions was examined under a variety of conditions including high and low substrate concentrations, at pH 4 and pH 7, with and without glutathione. At high insulin concentration (4.1 - 10(-5) M) insulin degradation by the homogenate was greatest at pH 4 but at low insulin concentration (1 - 10(-10) M) insulin degradation was greatest at pH 7. At either high or low glucagon concentration glucagon degradation by the homogenate was greatest at pH 7. Glutathione at pH 7 stimulated insulin degradation at high insulin concentrations and inhibited insulin degradation at low concentrations; Glucagon degradation at pH 7 was inhibited at both high and low concentrations of glucagon by glutathionemseparation of kidney into cortex and medulla prior to homogenation produced a pattern of insulin and glucagon degradation identical to the whole homogenate but glucagon degradation by the medulla was greater than by the cortex. Examination of degradation by subcellular fractions revealed that at high concentration at neutral pH most insulin was degraded by the 100 000 X g pellet but at low insulin concentrations over 90% of the activity was in the 100 000 X g supernatan...Continue Reading

Mentioned in this Paper

Adrenal Cortex Diseases
Kidney
Glucagon
Structure of Cortex of Kidney
Insulin B Chain
Subcellular Fractions
Hydrogen-Ion Concentration
Kidney Papilla
Gcg
Metazoa

About this Paper

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