Insulin and IGF binding by IGFBP-3 fragments derived from proteolysis, baculovirus expression and normal human urine

The Journal of Clinical Endocrinology and Metabolism
Peter VorwerkRon G Rosenfeld

Abstract

Recombinant human IGFBP-3 was proteolysed with different concentrations of plasmin for various periods of time. The major IGFBP-3 fragment resulting from this digestion migrated at ca. 15 kDa in nonreducing SDS-PAGE. Following the identification of this fragment as an N-terminal IGFBP-3 fragment, by use of N-terminus-specific monoclonal antibody and amino acid sequence analysis, we constructed and expressed a similar fragment in a baculovirus expression system. The fragments resulting from plasmin digestion, as well as the baculovirus-expressed recombinant human IGFBP-3(1-97), retain weak IGF binding and show specific insulin binding on cross-linking and western ligand blot. RhIGFBP-3(1-97) can inhibit insulin receptor autophosphorylation in insulin receptor-overexpressing NIH 3T3 cells. Insulin and IGF binding to IGFBP-3 fragments could be further demonstrated in normal urine. These data indicate the physiological significance of IGFBP-3 fragments derived from proteolysis in vivo.

Citations

Sep 1, 2004·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Steven A Rosenzweig
Sep 16, 2004·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Victoria Schebek-FürstenbergBernd Kübler
Nov 23, 2007·Cancer Detection and Prevention·Sonali PechlivanisAsta Försti
Feb 22, 2008·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Wensen ChenHongbing Shen
Dec 6, 2002·Endocrine Reviews·Sue M Firth, Robert C Baxter
Dec 13, 2003·Endocrine Reviews·Stephanie R EdmondsonChristopher J Wraight
Dec 22, 1999·Endocrine Reviews·V HwaR G Rosenfeld
Oct 16, 2001·The Journal of Clinical Endocrinology and Metabolism·C K BuckwayR G Rosenfeld
Mar 10, 1999·The American Journal of Physiology·B A BoothR S Bar
May 29, 2000·American Journal of Physiology. Endocrinology and Metabolism·R C Baxter
May 19, 2009·Molecular Biology Reports·Li LiKeke Huo
Mar 13, 2009·American Journal of Physiology. Cell Physiology·Paulette M Yamada, Kuk-Wha Lee
Oct 21, 1999·Molecular Genetics and Metabolism·L A WetterauP Cohen
Dec 12, 2001·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·G R DeviR G Rosenfeld
Jun 15, 2010·Archives of Biochemistry and Biophysics·Monalisa SwainHanudatta S Atreya

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.