Insulin and Insulin-like growth factor-1 can activate the phosphoinositide-3-kinase /Akt/FoxO1 pathway in T cells in vitro

Dermato-endocrinology
Yasaman MirdamadiHarald Gollnick

Abstract

Hyper-glycemic food increases insulin-like growth factor 1 (IGF-1) and insulin signaling and regulates endocrine responses and thereby may modulate the course of acne. Inflammation and adaptive immune responses have a pivotal role in all stages of acne. Recent hypothesis suggests that hyperglycemic food reduces nuclear forkhead box-O1 (FoxO1) transcription factor and may eventually induces acne. The aim of our study was to investigate the role of IGF-1 and insulin on the phosphoinositide-3-kinase (PI3K)/Akt/FoxO1 pathway in human primary T cells and on the molecular functions of T cells in vitro. T cells were stimulated with 0.001 μM IGF-1 or 1 μM insulin +/- 20 μM PI3K inhibitor LY294002. T cells were also exposed to SZ95 sebocyte supernatants which were pre-stimulated with IGF-1 or insulin. We found that 0.001 µM IGF-1 and 1 µM insulin activate the PI3K pathway in T cells leading to up-regulation of p-Akt and p-FoxO1 at 15 and 30 minutes. Nuclear FoxO1 was decreased and FoxO transcriptional activity was reduced. 0.001 µM IGF-1 and 1 µM insulin increased T cell proliferation but have no significant effect on Toll-like receptor2/4 (TLR) expression. Interestingly, supernatants from IGF-1- or insulin-stimulated sebocytes activate...Continue Reading

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Citations

Aug 26, 2018·Experimental Dermatology·Ge ShiYi-Ming Fan
Nov 30, 2018·Bioscience, Biotechnology, and Biochemistry·Yingze WeiXiaoxia Jin
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Feb 24, 2020·Dermatology : International Journal for Clinical and Investigative Dermatology·KeunOh ChoiYoungJoo Lee

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Methods Mentioned

BETA
biopsies
flow cytometry
transfection
fluorescence microscopy

Software Mentioned

Kodak 1D Image Analysis
Metaview

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