PMID: 6404679May 1, 1983Paper

Insulin and insulin-like growth factor I. Effects on protein synthesis in isolated muscles from lean and goldthioglucose-obese mice

Diabetes
S MonierY Le Marchand-Brustel

Abstract

The effects of insulin and insulin-like growth factor I (IFG-I) on protein synthesis were compared in muscle isolated from lean and goldthioglucose (GTG)-obese mice. Two types of skeletal muscles, the red soleus and the white extensor digitorum longus (EDL) muscles, were studied. In muscles from lean mice, 6.7 nM insulin and 50 nM IGF-I caused a similar maximal stimulation of tyrosine incorporation in total proteins (40% increase). However, the potency of IGF-I was only 5-10% that of insulin both in soleus and in EDL muscles (EC50 approximately equal to 6 nM for IGF-I and 0.5 nM for insulin). Basal rate of protein synthesis was identical in muscles from GTG-obese and lean mice. Similarly, a comparable increase in the rate of protein synthesis was obtained using maximally effective concentrations of insulin and IGF-I in both lean and GTG-obese animals. SDS-polyacrylamide gel electrophoresis analysis of proteins labeled with 35S-methionine confirmed that, in muscles from lean and GTG-obese animals, insulin and IGF-I increased overall protein synthesis in a similar manner. These results suggest that the protein synthesis machinery is not impaired in GTG-induced obesity, which is therefore not associated with resistance to insulin ...Continue Reading

Citations

Aug 1, 1984·Molecular and Cellular Endocrinology·S Monier, Y Le Marchand-Brustel
May 1, 1984·The Journal of Clinical Investigation·S Melmed
Apr 1, 1993·The Journal of Clinical Investigation·S J HeydrickY Le Marchand-Brustel
Dec 27, 2012·Disease Models & Mechanisms·Paolo Bonaldo, Marco Sandri
Apr 1, 1986·Diabetologia·G AugertY Le Marchand-Brustel
Jan 1, 1987·Tissue & Cell·M V DodsonD C McFarland
Jun 21, 2015·Cellular and Molecular Life Sciences : CMLS·David Sala, Antonio Zorzano
Mar 23, 2013·The FEBS Journal·Stefano SchiaffinoMarco Sandri
Sep 1, 1984·Diabetic Medicine : a Journal of the British Diabetic Association·R Taylor
May 1, 1991·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·R L BakerP D Gluckman

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