Insulin and insulin mutants stimulate glucose uptake in rat adipocytes

Science in China. Series C, Life Sciences
S YaoS Zhu

Abstract

A simple method to determine the in vitro biological activity of insulin by measuring glucose uptake in the rat adipocytes is presented here. In the presence of insulin, the glucose uptake is 5-6 times more than the basal control. And the uptake of D-[3-(3)H]-glucose is linear as the logarithm of insulin concentration from 0.2 microg/L to 1.0 microg/L. Glucose and 3-O-methyl-glucose inhibit D-[3-(3)H]-glucose uptake into adipocytes. By this method, the in vitro biological activity of [B(2)-Lys]-insulin and [B(3)-Lys]-insulin was measured to be 61.6% and 154% respectively, relative to that of insulin.

References

Mar 1, 1989·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·S ZeuzemK Schöffling
May 1, 1989·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·R TaylorS Tolley
Jan 1, 1974·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·A J MoodyJ Gliemann
Aug 1, 1996·Protein Science : a Publication of the Protein Society·G D SmithW Pangborn

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