Insulin and messenger ribonucleic acid expression of insulin receptor isoforms in ovarian follicles from nonhirsute ovulatory women and polycystic ovary syndrome patients

The Journal of Clinical Endocrinology and Metabolism
Jennifer L PhyDaniel A Dumesic

Abstract

Insulin action is mediated by two insulin receptor (IR) isoforms, differing in mitogenic and metabolic function. IR isoform expression might occur in human granulosa cells and could be altered in polycystic ovary syndrome (PCOS) from hyperinsulinemia. To determine the relationship between granulosa cell IR isoform expression and follicular fluid insulin concentration in individual follicles, 18 normal women and seven PCOS patients receiving gonadotropins for in vitro fertilization were studied. Glucose tolerance testing was performed before pituitary desensitization, and fasting serum insulin was measured at oocyte retrieval. Granulosa cells and fluid aspirated from the first follicle were used to determine IR isoform mRNA expression and insulin concentration, respectively. IR isoform A mRNA expression was greater than that of IR isoform B expression in normal mural granulosa and cumulus cells, without a cell type effect. Intrafollicular insulin levels increased with adiposity and serum insulin levels at oocyte-retrieval but did not predict IR mRNA expression. Total IR mRNA expression, but not intrafollicular insulin levels, was elevated in PCOS patients, whereas intrafollicular insulin levels were increased in women with impai...Continue Reading

Citations

Nov 18, 2011·Cell and Tissue Research·R N ChavesJ R Figueiredo
Jan 31, 2008·Expert Reviews in Molecular Medicine·Evanthia Diamanti-Kandarakis
Jan 26, 2011·Nature Reviews. Endocrinology·Mark O GoodarziRicardo Azziz
Dec 18, 2007·Obstetrical & Gynecological Survey·Daniel A DumesicDavid H Abbott
Nov 14, 2012·Therapeutic Advances in Endocrinology and Metabolism·Alexandra Bargiota, Evanthia Diamanti-Kandarakis
Jan 9, 2008·Seminars in Reproductive Medicine·Daniel A Dumesic, David H Abbott
Nov 6, 2013·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Tawiwan Pantasri, Robert John Norman
Dec 17, 2014·Fertility and Sterility·Daniel A DumesicWilliam B Schoolcraft
Mar 12, 2013·Fertility and Sterility·Daniel A Dumesic, Joanne S Richards
Mar 3, 2018·Journal of Assisted Reproduction and Genetics·M G Da BroiP A Navarro
Dec 7, 2006·The Journal of Clinical Endocrinology and Metabolism·Jennifer R WoodJerome F Strauss
Oct 30, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Francisco WestermeierLuis Sobrevia
Apr 21, 2020·Endocrine Reviews·Elisabet Stener-VictorinDavid H Abbott
Feb 19, 2009·The Journal of Clinical Endocrinology and Metabolism·Rebecca L RobkerRobert J Norman
May 29, 2016·The Biochemical Journal·Joëlle Dupont, Rex J Scaramuzzi
Oct 3, 2015·Diabetes/metabolism Research and Reviews·F WestermeierL Sobrevia
Mar 5, 2005·Reproduction, Fertility, and Development·Daniel A DumesicDavid H Abbott
Jun 17, 2021·European Journal of Clinical Nutrition·Bettina Hieronimus, Regina Ensenauer

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