Insulin attenuates endotoxin-induced acute lung injury in conscious rats

Critical Care Medicine
Hsing I ChenShang Jyh Kao

Abstract

To investigate the effects of insulin on the acute lung injury induced by lipopolysaccharide using a conscious rat model. Prospective, randomized, controlled animal study. University research laboratory. A total of 190 adult male Sprague-Dawley rats weighing 250-300 g. Endotoxemia was induced by intravenous infusion of lipopolysaccharide. Lipopolysaccharide at various doses (0, 1, 5, 10, 20, and 30 mg/kg, n=10 for each dose) was administered intravenously in 20 mins. Insulin infusion at doses of 0.5, 1, and 5 microU/kg/min was given 5 mins before lipopolysaccharide administration. Plasma glucose was clamped at 90-110 mg/dL by infusion of 10-80% glucose solution. Insulin and glucose infusion (0.01 mL/min) was started 5 mins before lipopolysaccharide and continued for 120 mins. The rats received a total of 60, 120, and 600 microU/kg insulin as well as 0.12, 0.36, and 0.96 g of glucose in respective groups. The animals were then observed for 4 hrs. The extent of acute lung injury was evaluated by lung weight/body weight ratio, lung weight gain, protein concentration in bronchoalveolar lavage, and exhaled nitric oxide. We also measured plasma nitrate/nitrite and methyl guanidine. In addition, histopathologic changes of the lung wer...Continue Reading

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Citations

Nov 26, 2010·Pharmaceutical Research·Sok Bee LimHayat Önyüksel
Jan 25, 2008·Critical Care Medicine·Demeral David LiuHsing I Chen
Apr 2, 2008·Critical Care Medicine·Huang-Ping Yu, Ying-Tung Lau
Jun 18, 2009·Critical Care Medicine·Shyoko Honiden, Michelle N Gong
Feb 28, 2008·The Journal of Trauma·Shang-Jyh Kao, Hsing I Chen
Jan 5, 2007·Clinical and Experimental Pharmacology & Physiology·Demeral D LiuHsing I Chen
Feb 28, 2007·Clinical and Experimental Pharmacology & Physiology·Chain Fa SuHsing I Chen
Apr 19, 2007·Clinical and Experimental Pharmacology & Physiology·Shang-Jyh KaoHsing I Chen
Jul 2, 2009·Clinical and Experimental Pharmacology & Physiology·Wann-Cherng PerngDeh-Ming Chang
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Sep 21, 2007·Clinical and Experimental Pharmacology & Physiology·Yen Chin LiuHsing I Chen
Apr 23, 2008·Pathology·Yung-Hsiang Hsu, Hsing I Chen
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Sep 21, 2021·Experimental and Therapeutic Medicine·Xu-Peng Wen, Qi-Quan Wan

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