Insulin augments GnRH-stimulated LHbeta gene expression by Egr-1

Molecular and Cellular Endocrinology
Colleen BuggsFredric Wondisford

Abstract

Previous studies have shown that insulin augments GnRH-stimulated LH synthesis and release from primary gonadotrophs. In this study, regulation of LHbeta gene expression by GnRH and insulin was examined in LbetaT2 cells. Endogenous LHbeta mRNA is stimulated 2.4-fold by insulin alone, 2.6-fold by GnRH alone, and 4.7-fold by insulin together with GnRH. This effect of insulin, like GnRH, mapped to sequences -140 to +1 in the mouse LHbeta gene. Insulin together with GnRH stimulates activity of an LHbeta-reporter gene 7.1-fold; whereas, GnRH alone or insulin alone stimulates the reporter activity 2.8- and 3.1-fold, respectively. Blocking the binding of Egr-1 to sequences -51 to -42 in the LHbeta gene inhibits effects of insulin and GnRH. Insulin together with GnRH increases Egr-1 mRNA levels and total Egr-1 binding to LHbeta DNA. These findings indicate that insulin may impact regulation of the reproductive axis at the level of the pituitary.

References

Feb 12, 1992·Molecular and Cellular Biochemistry·M Alexander-BridgesN Nasrin
Jan 1, 1995·Progress in Nucleic Acid Research and Molecular Biology·A Gashler, V P Sukhatme
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T SasaokaJ M Olefsky
Jan 24, 1998·Molecular Endocrinology·P TopilkoP Charnay
Mar 15, 2003·The Journal of Biological Chemistry·Lujian TanMary B Goldring
Aug 6, 2004·American Journal of Obstetrics and Gynecology·Christoph DornYoel Sadovsky

❮ Previous
Next ❯

Citations

Aug 7, 2012·The Journal of Biological Chemistry·David J ArriolaVarykina G Thackray
Mar 13, 2008·The Journal of Clinical Endocrinology and Metabolism·Mark A LawsonR Jeffrey Chang
Aug 21, 2014·Journal of Molecular Endocrinology·Pui-Sin ManDavid A Carter
Nov 28, 2012·Molecular and Cellular Endocrinology·Lacey L LindamanDjurdjica Coss
Feb 14, 2015·Molecular and Cellular Endocrinology·Danalea V Skarra, Varykina G Thackray
Mar 23, 2013·Molecular Endocrinology·Josefa AndradeMargaret A Shupnik
Jul 4, 2017·Reproduction, Fertility, and Development·Virginie MaillardJoëlle Dupont
Jan 11, 2008·Current Opinion in Endocrinology, Diabetes, and Obesity·Takara Stanley, Madhusmita Misra
Jan 26, 2008·Molecular Endocrinology·Travis B SalisburyJohn H Nilson
Sep 13, 2019·Endocrinology·Nancy M Lainez, Djurdjica Coss
Apr 27, 2017·The Journal of Veterinary Medical Science·Kiran PandeyHiroya Kadokawa
Feb 2, 2021·Journal of Neuroendocrinology·Maggie C EvansGreg M Anderson
Jul 1, 2021·Surgery for Obesity and Related Diseases : Official Journal of the American Society for Bariatric Surgery·Mohammad Reza EmamiShahab Alizadeh
Jul 28, 2009·Molecular and Cellular Endocrinology·Amy M NavratilMark A Lawson
Oct 18, 2007·Current Opinion in Endocrinology, Diabetes, and Obesity

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.