PMID: 7031415Dec 1, 1981Paper

Insulin binding and action in isolated rat hepatocytes: evidence for spare receptors

Metabolism: Clinical and Experimental
H J FrankP A Serbin

Abstract

An in vitro assay for insulin action on hepatocytes is described. The 125I-insulin binding and the effects of insulin on net 14C-glucose incorporation into glycogen were studied in suspensions of isolated hepatocytes from fed, 250 gram adult rats. Insulin doubled the basal value (mean +/- SEM) of 9.0 +/- 1.0 nmoles glucose/10(6) cells/hr with a one-half maximal concentration of 3 ng/ml (75 microU/ml) and a maximum effect between 10 and 20 ng/ml (250 microU/ml). Insulin binding was half-maximal at 40 ng/ml and maximal between 100 and 300 ng/ml. Thus, maximal stimulation occurred at approximately 35% of maximum binding implying that hepatocytes have spare receptors for insulin action on net incorporation of 14C-glucose into glycogen. This assay was then used to investigate the time course of activation of insulin action. Isolated hepatocytes were preincubated at 37 degrees C in the presence or absence of 40 ng/ml of insulin for 2, 15, or 30 min, washed, and then tested for action in fresh insulin-free media containing 14C-glucose. No activation was seen after 2 min, a partial activation after 15 min and maximum activation was seen only after a 30 min preincubation. Therefore, insulin activation of glucose incorporation into glyco...Continue Reading

References

Dec 1, 1977·Diabetologia·O AndersenS Gammeltoft
Aug 1, 1978·Diabetologia·A Le Cam, P Freychet
Nov 1, 1973·Biochemical and Biophysical Research Communications·P de MeytsM A Lesniak
May 1, 1974·Analytical Biochemistry·J A BerlinerR G Karjala
Apr 16, 1971·Biochemical and Biophysical Research Communications·P FreychetD M Neville
May 1, 1965·Analytical Biochemistry·E Van Handel
Sep 1, 1949·The American Journal of the Medical Sciences·W C STADIE, N HAUGAARD

❮ Previous
Next ❯

Citations

Nov 18, 2004·Biogerontology·Goran KorićanacNevena Ribarac-Stepić
May 3, 2005·The Journal of Clinical Investigation·Christoph BuettnerLuciano Rossetti
Jul 1, 1993·The American Journal of Physiology·R HovorkaR H Jones
Jan 31, 1983·Life Sciences·N Venkatesan, M B Davidson
Jun 1, 1984·Metabolism: Clinical and Experimental·M B Davidson
Dec 1, 1982·Metabolism: Clinical and Experimental·M B Davidson, N Venkatesan
Mar 7, 1991·Journal of Theoretical Biology·B J HammondG D Smith

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.