PMID: 4890253Jan 1, 1969Paper

Insulin biosynthesis in the rat: demonstration of two proinsulins

Proceedings of the National Academy of Sciences of the United States of America
J L Clark, Donald F Steiner

Abstract

Proinsulin, a single-chain precursor of insulin, has been detected in biosynthetic studies in vitro,(1-4) and in crystalline preparations of insulin from several species.(1, 5-7) It appears that proinsulin normally serves neither as a major storage nor secretory form of the hormone.(8) Its primary functions seems to be to facilitate the efficient formation of the disulfide bonds of insulin.(9)Although several species of fish have two insulins differing slightly in primary structure,(10-12) the rat is the only mammal where this is known to be the case.(13, 14) As the two rat insulins are nonallelic(14) and presumably coded by two distinct genes, a separate proinsulin would be expected to precede each insulin. We have demonstrated the biosynthesis of both insulins(15) and of a proinsulin corresponding to each in isolated islets of Langerhans, and have detected probable intermediates in the conversion of these proinsulins to insulin. Labeled peptide material corresponding to the free connecting segment of proinsulin (C-peptide) was detected in insulin-containing fractions isolated from incubated rat islets. It was noted that islets incubated in vitro secreted small amounts of newly synthesized proinsulin as well as the two insulin...Continue Reading

References

Oct 30, 1965·Nature·S L HowellK W Taylor
Jun 1, 1966·Endocrinology·I A Mirsky, K Kawamura
Jun 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·D F Steiner, J L Clark
Nov 17, 1967·Biochemical and Biophysical Research Communications·C C Yip, B J Lin
Jul 12, 1968·Science·R E ChanceW W Bromer
May 1, 1966·The American Journal of Medicine·L F Smith
Feb 21, 1967·Biochimica Et Biophysica Acta·K R Woods, K T Wang
Nov 1, 1960·Analytical Biochemistry·K A PIEZ, L MORRIS
Sep 10, 1962·Biochimica Et Biophysica Acta·P R DAVOREN

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Citations

Jan 1, 1986·Journal of Molecular Evolution·B M WentworthJ M Chirgwin
Jan 1, 1985·Journal of Molecular Evolution·M B SoaresA Efstratiadis
May 15, 1969·The New England Journal of Medicine·D F Steiner
Feb 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M A Permutt, D M Kipnis
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J W HamiltonD V Cohn
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·C C YipH Hsu
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S J ChanD F Steiner
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J R Duguid, D F Steiner
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L Villa-KomaroffW Gilbert
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S J ChanD F Steiner
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K KakitaM A Permutt
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L Muglia, J Locker
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·O D MadsenD F Steiner
Apr 2, 2011·The Journal of Biological Chemistry·Donald F Steiner
Mar 1, 1970·The Journal of Clinical Investigation·N R LazarusL Recant
Jul 1, 1970·The Journal of Clinical Investigation·T TaneseL Recant
Apr 1, 1972·The Journal of Clinical Investigation·A H RubensteinD F Steiner
May 1, 1976·The Journal of Clinical Investigation·A AnderssonJ Ostman
Oct 1, 1978·The Journal of Clinical Investigation·C de HaënR H Williams
Oct 1, 1985·The Journal of Clinical Investigation·B D GivenH S Tager
Aug 1, 1992·Molecular and Cellular Endocrinology·B M WentworthL Villa-Komaroff
Jun 1, 1970·Biochimica Et Biophysica Acta·G E Morris, A Korner
Feb 1, 1978·Archives of Biochemistry and Biophysics·S P WoodD F Steiner
Aug 1, 1983·General and Comparative Endocrinology·W B Rhoten
Nov 13, 1987·Biochemical and Biophysical Research Communications·S Nagamatsu, G M Grodsky
Dec 9, 1970·Biochemical and Biophysical Research Communications·W Kemmler, D F Steiner
Jan 1, 1980·Annals of the New York Academy of Sciences·T A RapoportS Rosenthal
Jun 1, 2000·Metabolism: Clinical and Experimental·K NodaN Toyoda
Nov 1, 1972·Clinics in Endocrinology and Metabolism·N R Lazarus
Aug 15, 2002·Biochemical and Biophysical Research Communications·Helen Roderigo-MilnePeter M Jones
May 21, 1999·The Journal of Biological Chemistry·U ShindeM Inouye
Jun 29, 1973·Nature·D F Steiner

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