Insulin deficiency induces rat renal mesangial cell dysfunction via activation of IGF-1/IGF-1R pathway

Acta Pharmacologica Sinica
Ya-li KongLimin Lu

Abstract

Diabetic nephropathy is one of the major complications of diabetes and the major cause of end-stage renal disease. In this study we investigated the insulin deficiency (ID) induced changes in renal mesangial cells (MCs) and in the kidney of STZ-induced diabetic rats. Cultured rat renal MCs were incubated in ID media. Cell proliferation was analyzed using BrdU incorporation assay. The expression of insulin receptor (IR), insulin-like growth factor-1 receptor (IGF-1R), phosphorylated IGF-1R, fibronectin, and collagen IV was determined with Western blot analysis. STZ-induced diabetic rats were treated with an IGF-1R antagonist picropodophyllin (PPP, 20 mg·kg(-1)·d(-1), po) for 8 weeks. After the rats were euthanized, plasma and kidneys were collected. IGF-1 levels in renal cortex were measured with RT-PCR or ELISA. The morphological changes in the kidneys were also examined. Incubation in ID media significantly increased cell proliferation, the synthesis of fibronectin and collagen IV, and the expression of IGF-1 and IGF-1R and phosphorylated IGF-1R in renal MCs. Pretreatment of the cells with PPP (50 nmol/L) blocked ID-induced increases in cell proliferation and the synthesis of fibronectin and collagen IV; knockdown of IGF-1R sh...Continue Reading

References

Apr 1, 1989·The Journal of Diabetic Complications·P M ter Wee, J M Donker
Jul 3, 2004·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Ralph Rabkin, Franz Schaefer
Dec 25, 2007·Experimental Biology and Medicine·Yashpal S KanwarFarhad R Danesh
Dec 17, 2008·Current Topics in Medicinal Chemistry·Stephen L Gwaltney
Apr 17, 2009·American Journal of Physiology. Renal Physiology·Jin Ji LiShin-Wook Kang
Jun 17, 2009·Journal of Cardiovascular Pharmacology·Pitchai BalakumarMadhu B Anand-Srivastava
Sep 24, 2010·Kidney International·János Peti-Peterdi
Jan 7, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Ping YuanLimin Lu
Jun 12, 2012·Endocrinology and Metabolism Clinics of North America·Masanobu Kawai, Clifford J Rosen
Jun 12, 2012·Endocrinology and Metabolism Clinics of North America·S John Weroha, Paul Haluska
Jul 31, 2012·BMC Nephrology·Eric W Brunskill, S Steven Potter
Aug 11, 2012·Acta neurologica Belgica·Saida HaiderDarakhshan J Haleem
Oct 30, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S O KolsetT Jenssen
Nov 6, 2012·Journal of the American Society of Nephrology : JASN·Youli WangYan Chun Li
Mar 25, 2014·Current Drug Targets·Mandeep Kumar Arora, Umesh Kumar Singh
Aug 22, 2014·Diabetology & Metabolic Syndrome·Kei FukamiJosephine M Forbes
Dec 3, 2014·Pharmacology, Biochemistry, and Behavior·Amir-Hossein Bayat, Abbas Haghparast

❮ Previous
Next ❯

Citations

Sep 14, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ying BaiSihua Gao
Nov 1, 2016·Nature Reviews. Nephrology·Ferruh ArtuncHans-Ulrich Häring
Jun 5, 2019·Bioscience Reports·Liang RenShengqiang Wang
Jul 31, 2019·American Journal of Physiology. Endocrinology and Metabolism·Rong DongYan Zha

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.