PMID: 6991313Jan 1, 1980Paper

Insulin degradation by mononuclear cells

Diabetes
A C PowersW C Duckworth

Abstract

Mononuclear cells from peripheral blood possess insulin receptors that are altered in number or binding affinity in certain metabolic diseases as obesity. The monocyte, and not the lymphocyte, is the cell with the capacity to specifically bind insulin. Furthermore, this binding appears to mirror the receptor status on such insulin target tissues as liver, muscle, and fat. Since liver, muscle, and fat also degrade insulin, mononuclear cells from the blood of normal volunteers were examined for insulin-degrading activity. Intact cells were incubated with 125I-insulin and the amount of degraded insulin was measured by the trichloroacetic acid-precipitation technique. Insulin-degrading activity increased when the number of cells and the time of incubation were increased. Total insulin binding behaved in a similar fashion. Very little degradation was seen at 4 degrees or 15 degrees. The Km for insulin-degrading activity was 7.03 X 10(-8) M. Homogenized mononuclear cells degraded two to five times more insulin than did intact cells and also demonstrated cell concentration, time, and temperature dependence for degradation. The Km of degradation for homogenized mononuclear cells was 2.2 X 10(-8) M. Subcellular fractionation revealed si...Continue Reading

Citations

Nov 1, 1983·Diabetologia·G W NealW C Duckworth
Apr 10, 2007·Archivum Immunologiae Et Therapiae Experimentalis·Paweł PiatkiewiczJan Tatoń
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R K AssoianH S Tager
Oct 12, 2000·Clinical Chemistry and Laboratory Medicine : CCLM·J L PerretC Nguemby-Mbina
Jul 24, 2013·Journal of the American Podiatric Medical Association·Jérôme PatryMarie-Ludivine Chateau-Degat
Nov 24, 1982·Biochimica Et Biophysica Acta·G W Neal, A E Kitabchi
Oct 12, 1981·Biochimica Et Biophysica Acta·J M ConlonK G Alberti
Jul 1, 1982·Metabolism: Clinical and Experimental·A ChaitW Hammond
Dec 1, 1988·Immunological Reviews·T L DelovitchM L Phillips
Oct 30, 1998·Endocrine Reviews·W C DuckworthF G Hamel

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