Insulin dependence of M2 pyruvate kinase in primary culture of human liver

P GaliL Hartmann


In the basal state, the presence of L pyruvate kinase (LPK) was constantly observed in primary human liver cell cultures initiated from explants, when cells were examined by immunofluorescence and double labelling. After short-term insulin incubation, M pyruvate kinase (MPK) appeared. Therefore, both LPK and MPK were located in the same cells. We previously obtained the same results in isolated rat hepatocytes in which we demonstrated that short-term regulation of M2PK by insulin was a function of dose and/or incubation time. The present work established that similar conditions govern the regulation of this isozyme in vitro in human hepatocytes.


Jan 1, 1974·Biochimie·F LemonnierM Gautier
Jul 5, 1983·Biochimica Et Biophysica Acta·O MiyanagaG L Cottam
Sep 1, 1983·European Journal of Biochemistry·I Probst, Kurt Jungermann
Oct 12, 1984·Biochimica Et Biophysica Acta·W E FleigH Ditschuneit
Mar 28, 1980·Biochemical and Biophysical Research Communications·C Guguen-GuillouzoA Kahn
Apr 15, 1982·The Biochemical Journal·G P PooleD P Bloxham
Oct 30, 1981·Biochemical and Biophysical Research Communications·S OdashimaY Nagao
Sep 1, 1980·Journal of Cellular Physiology·F LemonnierA Lemonnier

Related Concepts

Cholestasis, Extrahepatic
Enzyme Activation
Immunofluorescence Assay
Insulin B Chain
R-Type Pyruvate Kinase

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