PMID: 7025655Sep 1, 1981Paper

Insulin does not hyperpolarize rat muscle by means of a ouabain-inhibitable process

The American Journal of Physiology
K Zierler, E M Rogus

Abstract

Experiments were designed to test the hypothesis that insulin-induced hyperpolarization of rat skeletal muscle is mediated by stimulation of a ouabain-inhibitable electrogenic pump. Parallel experiments were carried out on rat caudofemoralis with isoproterenol, known to hyperpolarize rat skeletal muscle by stimulation of such a pump. Ouabain (10(-5) M) completely inhibited isoproterenol-induced hyperpolarization within 15 min but had no effect on half-maximal insulin-induced hyperpolarization. Ouabain (10(-6) M) inhibited isoproterenol effect by 60% during a period of 5-15 min. Ouabain (10(-4) M) had no effect on insulin-induced hyperpolarization within 10 min but depolarized during the next 10 min. In a separate series of studies in rat extensor digitorum longus muscle, 10(-5) M ouabain increased intracellular Na+ within 14 min. It is concluded that in rat caudofemoralis muscle, insulin-induced hyperpolarization is not mediated by a ouabain-inhibitable electrogenic pump.

References

Jan 21, 1977·Biochimica Et Biophysica Acta·E M RogusK Zierler
Sep 1, 1977·The Journal of Physiology·T Clausen, J A Flatman
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R C LantzR L DeHaan
Feb 6, 1981·Biochimica Et Biophysica Acta·K Zierler, E M Rogus
Sep 1, 1959·The American Journal of Physiology·K L ZIERLER

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Citations

Jun 1, 1984·Journal of Cellular Physiology·H H Vandenburgh, C M Lent
Dec 29, 2000·American Journal of Veterinary Research·C BuranakarlK C Bovée
Sep 22, 1986·Life Sciences·M P GuptaN S Dhalla
Mar 21, 1983·Biochimica Et Biophysica Acta·R D Moore
Jan 1, 1988·Comparative Biochemistry and Physiology. A, Comparative Physiology·Y Marunaka
Feb 13, 1986·Biochemical and Biophysical Research Communications·A ZorzanoN B Ruderman
Nov 15, 1983·Biochemical and Biophysical Research Communications·P J Reeds, R M Palmer

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