PMID: 6162484Jan 20, 1981Paper

Insulin gene expression during development of the fetal bovine pancreas

Biochemistry
M L FrazierG F Saunders

Abstract

Poly(A+) RNA was isolated from the bovine pancreas at three stages of fetal development. Approximately 1% of the total RNA from first, second, and third trimester fetuses was polyadenylated, and the mean chain length of each RNA population was 1350 nucleotides. In cell-free protein synthesis experiments the concentration of insulin-immunoreactive translation products was 10.2%, 11.3%, and 9.7% for first, second, and third trimesters, respectively. Insulin mRNA sequences were estimated by transcription of insulin mRNA to [3H]cDNA and hybridization of cDNA with plasmid pI19 DNA containing rat proinsulin I sequences. Hybridization experiments gave insulin mRNA concentrations of 7.6%, 12.9%, and 3.9% for first, second, and third trimesters, respectively. These results show that insulin mRNA levels vary during development and become proportionally lower in third trimester, when the exocrine tissue is rapidly increasing in mass.

References

Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L Villa-KomaroffW Gilbert
Jun 1, 1977·Nucleic Acids Research·W R PearsonR J Britten
Nov 11, 1977·Science·P T Lomedico, G F Saunders
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M Tien KuoG F Saunders
Feb 1, 1976·Nucleic Acids Research·P T Lomedico, G F Saunders
Jan 1, 1974·Methods in Enzymology·D L Kacian, S Spiegelman
Oct 1, 1968·Journal of Cellular Physiology·W J RutterT G Sanders

❮ Previous
Next ❯

Citations

Jan 1, 1988·Biulleten' eksperimental'noĭ biologii i meditsiny·A G Iakovlev
Apr 15, 1988·Biochemical and Biophysical Research Communications·J KudoG F Saunders
Feb 26, 2000·European Journal of Biochemistry·E Fernández-SalasR de Llorens
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M TashimaG F Saunders
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T Shiosaka, G F Saunders
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L C LopezG F Saunders
Nov 15, 1988·Biochemical and Biophysical Research Communications·L C Lopez, B D Shur
Feb 1, 1996·International Journal of Pancreatology : Official Journal of the International Association of Pancreatology·M L FrazierD B Evans

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
M L FrazierB Levin
Differentiation; Research in Biological Diversity
M T KuoG F Saunders
Proceedings of the National Academy of Sciences of the United States of America
T Shiosaka, G F Saunders
© 2021 Meta ULC. All rights reserved