PMID: 9163577Mar 12, 1997Paper

Insulin gene expression in immortalized rat hippocampal and pheochromocytoma-12 cell lines

Regulatory Peptides
B S SinghS U Devaskar

Abstract

Employing reverse transcription-polymerase chain reaction and clonal cell lines derived by retroviral transduction of the temperature sensitive simian virus 40 large T-antigen into dispersed rat embryonic hippocampal cells, we detected the ancestral gene-insulin II mRNA in three progenitor subcloned cell lines. These cell lines upon differentiation are known to express markers indicative of commitment to either neuronal (H19-7; NF + , GFAP -), glial (H19-5; GFAP +, NF -), or bipotential (H583-5, NF +, GFAP + ) lineages. No duplicated, i.e., insulin I gene expression, was observed in any of the three cell lines. Induction of differentiation was associated with the persistence of insulin II mRNA and in the cells expressing a neuronal phenotype (H19-7; NF +, GFAP -) a relative doubling in insulin II mRNA level was present (P < 0.05). Minimal cellular insulin immunoreactivity was detected only in a subpopulation of cells with a differentiated neuronal phenotype. Radioimmunoassayable insulin peptide in the H19-7 cellular conditioned medium revealed a 5-fold increase in the differentiated state. In contrast, peripheral sympathetic PC-12 neuronal cells both in the undifferentiated and nerve growth factor-driven differentiated states, ...Continue Reading

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Citations

Sep 8, 2012·Molecular Neurobiology·Rasoul GhasemiAbolhassan Ahmadiani
Apr 5, 2001·Brain Research. Developmental Brain Research·R Schechter, M Abboud
Oct 16, 1999·Brain Research. Developmental Brain Research·R SchechterG Johnson
May 30, 2001·Molecular and Cellular Endocrinology·W Q Zhao, D L Alkon
Feb 26, 2004·Lancet Neurology·Suzanne Craft, G Stennis Watson
Mar 22, 2014·Neuroscience Bulletin·Yanxing ChenCheng-Xin Gong
Sep 24, 2008·Biochemical and Biophysical Research Communications·Golnaz MadadiDenise D Belsham
Apr 25, 2006·Journal of the Neurological Sciences·G Stennis Watson, Suzanne Craft
Jul 9, 2010·Trends in Endocrinology and Metabolism : TEM·Jakob Suckale, Michele Solimena
Sep 19, 2012·Stem Cells International·Masanao MachidaTomoko Kuwabara
Jan 17, 2013·Therapeutic Advances in Endocrinology and Metabolism·Katherine Samaras, Perminder S Sachdev
Mar 11, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Dianne P Figlewicz
Jan 4, 2020·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Bhavani KashyapWilliam H Frey Ii
Sep 3, 2019·Frontiers in Neuroscience·Francesca FioryFrancesco Oriente

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