Insulin glulisine--a comprehensive preclinical evaluation

International Journal of Toxicology
Ingo StammbergerThomas Bartels

Abstract

Receptor binding and signaling and the mitogenic potential of insulin glulisine (glulisine), regular human insulin (RHI), and Asp(B10) were compared in vivo and in vitro. Insulin and insulin-like growth factor 1 (IGF-1) receptor binding was studied with human insulin receptors (293HEK cells) and the human osteosarcoma-derived cell line B10. Insulin receptor-mediated signaling was assessed in rat-1 fibroblasts overexpressing insulin receptors. Activation of insulin receptor substrates 1 and 2 (IRS-1/ IRS-2) was studied in rat and human myoblasts and rat cardiomyocytes. DNA synthesis induction was assessed by [3H] thymidine incorporation in the human epithelial breast cell line MCF10. Interaction with the IGF-1 receptor, DNA synthesis, and intracellular signal transduction were assessed in cardiac K6 myoblasts. Immunohistochemical examination of Sprague-Dawley rat tissue treated with glulisine for 6 months (n = 40), and glulisine and RHI for 12 months (n = 60), was performed. Steady-state insulin receptor binding affinity was slightly lower for glulisine versus RHI (approximately 0.70). IGF-1 receptor binding affinity was lower (four- to fivefold) for glulisine, but significantly higher (four-fold) for Asp(B10) versus RHI. Glulis...Continue Reading

References

Jan 2, 1992·International Journal of Cancer. Journal International Du Cancer·G PillemerD Naor
May 5, 1998·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·L BertiH U Häring
Oct 11, 2003·Biochemical and Biophysical Research Communications·Irini RakatziJürgen Eckel
Jan 29, 2005·Diabetes·Anita M HennigeHans-Ulrich Häring
Nov 26, 2005·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·M DreyerR Van Leendert

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Citations

Feb 24, 2007·Diabetes Technology & Therapeutics·Reinhard H A Becker
May 25, 2011·Endocrine-related Cancer·Antonino Belfiore, Roberta Malaguarnera
Mar 16, 2012·Endocrine-related Cancer·Aimee J Varewijck, Joseph A M J L Janssen
Jan 1, 2010·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Anna LihJohn N Carter
Oct 6, 2014·The American Journal of Medicine·Joseph M Tibaldi
Feb 17, 2009·Expert Opinion on Biological Therapy·Jason L Iltz
Dec 22, 2006·Expert Opinion on Drug Safety·Ali D Kamal, Stephen C Bain
Jun 3, 2009·Archives of Physiology and Biochemistry·Juergen Sandow
May 10, 2008·Archives of Physiology and Biochemistry·Ernst ChantelauRolf Meyer-Schwickerath
May 10, 2008·Archives of Physiology and Biochemistry·Kristin Eckardt, Jürgen Eckel
May 10, 2008·Archives of Physiology and Biochemistry·Doris MayerHarald Enzmann
Oct 19, 2010·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Henning HvidHenrik E Jensen
Apr 14, 2016·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Katie L AndersonOlivier Thibault
Aug 2, 2014·Clinical Therapeutics·Jen-Chieh LinMei-Shu Lai
Jun 7, 2014·Frontiers in Endocrinology·Joseph A M J L Janssen, Aimee J Varewijck
Apr 2, 2009·The Annals of Pharmacotherapy·Kristen L Helms, Kristi W Kelley
Jul 30, 2020·International Journal of Toxicology·Vivi Flou Hjorth JensenAnne-Marie Mølck
Jun 2, 2009·Archives of Physiology and Biochemistry·Ashish ShuklaDoris Mayer

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Methods Mentioned

BETA
density gradient centrifugation

Software Mentioned

ease
GraphPad Prism
Prism (
Prism
ease ( ISI )
GraphPad )

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