Insulin growth factor promotes human corneal fibroblast network formation in vitro

Investigative Ophthalmology & Visual Science
Alexandre BerthautM Mirshahi

Abstract

Corneal fibroblast cell (CFC) reticulation is involved in the structural development of corneal stroma and in wound healing. In an earlier paper, it was reported that the expression of VEGFR-1 by CFCs is related to their reticulogenic properties in vitro and decreases with the age of the donors. The present study was focused on the nonreticulogenic corneal fibroblast population and explored whether these cells can be induced to form cell networks in vitro. The network formation was analyzed using an array of signaling pathway inhibitors: wortmannin for PI3 kinase, U0126 for MEK-1/2 kinase, Rottlerin for PKC, farnesyl transferase inhibitor (FTI-277) for Ras, and picropodophyllin (PPP) for IGFR-1. Among the several growth factors studied, IGF seemed to be crucial to cell network formation. The presence of IGF signaling was demonstrated using gene array analysis and was confirmed by RT-PCR and immunocytochemistry and by cell network formation on reduced synthetic basement membrane arrays. The pleiotropic effect of IGF-1 on the cells was analyzed by protein cytokine array. The genesis of reticulation was found to occur via MEK-1/2 and IGFR pathways, since inhibitors of these signaling pathways reduced or prevented cell network form...Continue Reading

Citations

Jul 16, 2014·Archives of Gynecology and Obstetrics·Ayse Gul KebapcilarCetin Celik
Jul 21, 2015·Experimental Eye Research·Boris Hinz
Mar 16, 2013·American Journal of Medical Genetics. Part a·Karen W Gripp, Laurie A Demmer
Mar 31, 2015·Experimental Eye Research·W Matthew Petroll, Miguel Miron-Mendoza
Feb 4, 2014·The British Journal of Ophthalmology·Ahmet OzkokPinar Kadioglu
Mar 21, 2020·Frontiers in Endocrinology·Whitney L StuardDanielle M Robertson
Aug 18, 2021·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Deniz KilicGuzide Gonca Oruk

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