Insulin increases excitability via a dose-dependent dual inhibition of voltage-activated K+ currents in differentiated N1E-115 neuroblastoma cells

The European Journal of Neuroscience
Pedro A LimaPedro F Costa

Abstract

A role in the control of excitability has been attributed to insulin via modulation of potassium (K(+)) currents. To investigate insulin modulatory effects on voltage-activated potassium currents in a neuronal cell line with origin in the sympathetic system, we performed whole-cell voltage-clamp recordings in differentiated N1E-115 neuroblastoma cells. Two main voltage-activated K(+) currents were identified: (a) a relatively fast inactivating current (I(fast) - time constant 50-300 ms); (b) a slow delayed rectifying K(+) current (I(slow) - time constant 1-4 s). The kinetics of inactivation of I(fast), rather than I(slow), showed clear voltage dependence. I(fast) and I(slow) exhibited different activation and inactivation dependence for voltage, and have different but nevertheless high sensitivities to tetraethylammonium, 4-aminopyridine and quinidine. In differentiated cells - rather than in non-differentiated cells - application of up to 300 nm insulin reduced I(slow) only (IC(50) = 6.7 nm), whereas at higher concentrations I(fast) was also affected (IC(50) = 7.7 microm). The insulin inhibitory effect is not due to a change in the activation or inactivation current-voltage profiles, and the time-dependent inactivation is also...Continue Reading

References

May 1, 1978·The Journal of Physiology·W H Moolenaar, I Spector
Jan 1, 1988·The Journal of Physiology·F N Quandt
Dec 1, 1967·The Journal of Clinical Investigation·M J Perley, D M Kipnis
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M C Fishman, I Spector
Mar 1, 1983·Endocrinology·D G BaskinD M Dorsa
Dec 16, 1994·Brain Research. Developmental Brain Research·P F CostaM A Ribeiro
May 1, 1993·American Journal of Epidemiology·M Laakso
Aug 7, 1998·Brain Research. Developmental Brain Research·A I SantosP F Costa
Jul 22, 1999·Annals of the New York Academy of Sciences·B Rudy
Jul 22, 1999·Annals of the New York Academy of Sciences·W A CoetzeeB Rudy
Dec 11, 1999·Archives of General Psychiatry·S CraftR C Veith
Mar 14, 2000·Physiology & Behavior·C R ParkS C Woods
Oct 18, 2000·European Journal of Pharmacology·H L FehmJ Born
Dec 19, 2000·Neuroscience and Biobehavioral Reviews·R J SchulingkampR B Raffa
Apr 6, 2001·Diabetes Care·K A McAuleyA W Duncan
May 30, 2001·Molecular and Cellular Endocrinology·W Q Zhao, D L Alkon
Oct 16, 2001·Journal of Neurophysiology·M S Horning, P Q Trombley
Jun 27, 2002·Trends in Pharmacological Sciences·Laura GaspariniHuaxi Xu
Jul 11, 2002·Brain Research. Molecular Brain Research·Jonathan D RothSteven J Fluharty
Nov 7, 2003·The Journal of Clinical Endocrinology and Metabolism·Mary F CarrollDavid S Schade
Dec 31, 2003·Receptors & Channels·P FriederichB W Urban
Feb 19, 2004·Nutrition Reviews·Lais U Monzillo, Osama Hamdy
Feb 26, 2004·Lancet Neurology·Suzanne Craft, G Stennis Watson
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jianchao XuGary V Desir
Jan 29, 2005·The Journal of Physiology·Wiebke HirdesJürgen R Schwarz
Sep 1, 2005·Apoptosis : an International Journal on Programmed Cell Death·T S SchwabE L Feldman
Dec 15, 2006·Neuroendocrinology·Fuad Lechin, Bertha van der Dijs

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.