Insulin-induced egr-1 and c-fos expression in 32D cells requires insulin receptor, Shc, and mitogen-activated protein kinase, but not insulin receptor substrate-1 and phosphatidylinositol 3-kinase activation.

The Journal of Biological Chemistry
S HaradaL Jarett

Abstract

Many studies suggest that insulin utilizes multiple signal transduction pathways. Insulin's effects are initiated by insulin binding to the insulin receptor, resulting in tyrosine phosphorylation of insulin receptor and intracellular substrates, such as insulin receptor substrate-1 (IRS-1), IRS-2, or Shc. We recently demonstrated that immediate-early gene egr-1 transcription was fully induced without phosphorylation of IRS-1 in Chinese hamster ovary cells (Harada, S., Smith, R. M., Smith, J. A., Shah, N. , Hu, D.-Q. & Jarett, L. (1995) J. Biol. Chem. 270, 26632-26638). In the present study, we examined the effects of insulin on immediate-early gene egr-1 and c-fos expression in 32D cells overexpressing the insulin receptor (32D/IR), IRS-1 (32D/IRS), or both (32D/IR+IRS) and compared these effects with insulin-induced tyrosine phosphorylation. Insulin (17 nM) increased egr-1 and c-fos expression in 32D/IR and 32D/IR+IRS cells, but not in parental cells or 32D/IRS cells, as determined by Northern blot analysis. Insulin treatment (5 min at 37 degrees C) markedly increased tyrosine phosphorylation of several proteins, including the insulin receptor, IRS-1, and Shc, in 32D/IR+IRS cells as determined by immunoprecipitation and Wester...Continue Reading

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Citations

Apr 4, 2000·Molecular Carcinogenesis·P K RiggsJ DiGiovanni
Sep 26, 2006·Canadian Journal of Physiology and Pharmacology·Catherine Mounier, Barry I Posner
Oct 2, 2009·Future Oncology·Delphine Gitenay, Véronique T Baron
Jan 31, 2004·Annals of the New York Academy of Sciences·Véronique BaronDan Mercola
Oct 19, 1999·Microscopy Research and Technique·T TsakiridisG P Downey
Jan 14, 1999·The American Journal of Physiology·D M PitterleP J Blackshear
Oct 8, 1999·Archives of Biochemistry and Biophysics·B T SolowS Harada
Jul 25, 2021·Biochemical Pharmacology·Gerald ThielOliver G Rössler

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