Insulin-induced oxidative stress in the brain is nitric oxide-dependent

Pathophysiology : the Official Journal of the International Society for Pathophysiology
Isyaku U YarubeIsma'il A Umar

Abstract

Insulin is known to increase brain nitric oxide (NO) level and to cause oxidative stress but the relationship between these phenomena has not been well elucidated. This study aimed to examine the role of NO in the insulin-NO-oxidative stress axis in the brain. Mice were grouped into four (n = 5) and treated for seven days with 0.2 ml deionized water (control); 10 I.U./kg insulin; 10 I.U./kg insulin + 50 mg/kg L-NAME; and 50 mg/kg L-NAME. The mice were anaesthesized using ketamine + xylazine and sacrificed at the end of the study. Forebrain was immediately harvested from which brain homogenates were prepared in order to determine NO and malondialdehyde (MDA) concentrations as well as glutathione peroxidase (GPx) activity using commercially available kits. Data were processed using IBM SPSS Statistics 20.0. Nitric oxide values were higher in the insulin group (p < 0.05) but not in the insulin+L-NAME (p > 0.05) group when compared with the control. Values of MDA in the insulin and insulin+L-NAME groups were higher (p < 0.05) and the same (p > 0.05), respectively, than those in the control group. The activity of GPx in the insulin group was lower (p < 0.05) than, but that of the insulin+L-NAME was the same (p > 0.05) as in the cont...Continue Reading

Citations

Feb 20, 2020·Archives of Physiology and Biochemistry·Ahmet S YalçınkayaRagıp Balahoroğlu

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