Insulin induction of pip 92, CL-6, and novel mRNAs in rat hepatoma cells

Endocrine
K D BortoffJ L Messina

Abstract

Insulin directly affects many aspects of cellular metabolism. An additional, poorly studied effect of insulin is the regulation of multiple genes whose products are important in many cellular functions. Using differential screening techniques, we identified insulin-regulated genes induced in insulin-treated rat H4IIE (H4) hepatoma cells. Two of the mRNAs identified were homologous to the previously characterized mouse pip 92 and rat CL-6 immediate-early genes. The pip 92 clone was initially isolated from mitogen-stimulated mouse Balb/c 3T3 fibroblasts, whereas the CL-6 clone was first isolated from regenerating rat liver. In this article, we demonstrated that in rat H4 cells, the transcription rates of both pip 92 and CL-6 are induced by insulin alone. Additionally, we showed that the transcription rates of two other genes, whose sequences are not homologous to any other sequences in gene bank ("novel genes"), were rapidly and transiently induced by insulin. These results demonstrate that insulin regulates the expression of several novel genes with a time-course similar to members of the immediate-early response gene family.

References

Jan 1, 1992·Annual Review of Physiology·S J Pilkis, D K Granner
Jun 1, 1992·Experimental Cell Research·J L Messina, R S Weinstock
Mar 1, 1992·Molecular and Cellular Endocrinology·K D Bortoff, J L Messina
Jan 1, 1992·Molecular Endocrinology·J L Messina
Jan 1, 1991·Annual Review of Biochemistry·H R Herschman
Sep 15, 1991·The Biochemical Journal·R M O'Brien, D K Granner
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J C HsuR Taub
Oct 30, 1990·Biochemical and Biophysical Research Communications·J L Messina, J A McCann
Dec 1, 1990·Molecular and Cellular Biology·C H CharlesL F Lau
Aug 20, 1990·Molecular and Cellular Endocrinology·R S Weinstock, J L Messina
Sep 1, 1985·Pediatric Research·D J Hill, R D Milner
Sep 18, 1987·Science·O M Rosen
Jan 1, 1987·Journal of Cellular Physiology. Supplement·T E HayesB H Cochran
Feb 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·B J Rollins, C D Stiles
Jul 1, 1988·Endocrinology·R S Weinstock, J L Messina
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L F Lau, D Nathans
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D J Stumpo, P J Blackshear
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P K ElderM J Getz
Feb 1, 1982·The Journal of Clinical Endocrinology and Metabolism·J M Podskalny, C R Kahn
Nov 1, 1994·Trends in Biochemical Sciences·R J Davis
Jan 1, 1994·Annual Review of Physiology·S R KimballL S Jefferson
Aug 1, 1994·Journal of Cellular Physiology·J L Messina
Feb 1, 1996·Molecular and Cellular Biology·K H HoltJ E Pessin
Mar 15, 1996·The Journal of Biological Chemistry· Dong ChenJ E Pessin
Apr 26, 1996·The Journal of Biological Chemistry·K A Heidenreich, J L Kummer
Nov 29, 1996·The Journal of Biological Chemistry·C M MoxhamC C Malbon

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Citations

Jul 10, 2003·Molecular and Cellular Endocrinology·Susan E Stred, Joseph L Messina
Jul 10, 2003·Molecular and Cellular Endocrinology·Susan E StredJoseph L Messina
Sep 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Bethany A Janowski
Apr 29, 2008·Brain Research Reviews·Megumi Sugahara KobayashiYasuo Takahashi
Dec 13, 2006·Journal of Neurochemistry·Jung Bum ParkKwang Chul Chung
Sep 11, 2004·Biochimica Et Biophysica Acta·Adam B KeetonJoseph L Messina
Apr 19, 2016·Biochemical and Biophysical Research Communications·J Lee FranklinJoseph L Messina
Feb 17, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Daoguang YanVesa M Olkkonen
Oct 26, 2001·The Journal of Biological Chemistry·Chandan Kumar-SinhaArul M Chinnaiyan
Oct 5, 2002·The Journal of Biological Chemistry·Adam B KeetonJoseph L Messina

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