Insulin inhibition of proteasome activity in intact cells

Biochemical and Biophysical Research Communications
Frederick G HamelWilliam C Duckworth

Abstract

Cellular homeostasis requires regulation of protein turnover. Protein degradation is an essential component of this process and is inhibited by insulin. The importance of cytosolic proteolysis in overall cellular protein degradation is increasingly apparent and an insulin effect on this system has been suggested but not proven. The present study shows that a membrane permeable substrate of the proteasome is degraded in HepG2 cells and that insulin inhibits its degradation both by isolated proteasomes and by intact cells. Inhibitors of the proteasome suppress degradation, and in the presence of these inhibitors insulin has no further effect. This is the first demonstration that insulin inhibition of cellular protein degradation is due to an effect on proteasomes.

References

Dec 1, 1992·The Journal of Clinical Investigation·R J LouardE J Barrett
Sep 15, 1991·The Biochemical Journal·R M O'Brien, D K Granner
Feb 1, 1989·Diabetes/metabolism Reviews·G E MortimoreB R Lardeux
Jan 1, 1980·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·F J Ballard, J M Gunn
Nov 1, 1980·Journal of Cellular Physiology·F J BallardJ M Gunn
Apr 1, 1993·The Biochemical Journal·A J Rivett
Apr 1, 1995·The American Journal of Physiology·P Q BarrettC J Cohen
Jul 29, 1994·Biochemical and Biophysical Research Communications·R G BennettW C Duckworth

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Citations

Jan 26, 2002·Molecular and Cellular Biology·Rachel ZhandeXiao Jian Sun
Dec 4, 1998·Bioscience, Biotechnology, and Biochemistry·T NagasawaN Nishizawa
May 15, 2002·European Journal of Pharmacology·Geert Jan BiesselsWillem Hendrik Gispen
Jul 19, 2003·Metabolism: Clinical and Experimental·Frederick G HamelWilliam C Duckworth
May 9, 2009·Metabolism: Clinical and Experimental·Frederick G Hamel
Sep 27, 2005·The International Journal of Biochemistry & Cell Biology·Terrence M DonohueDahn L Clemens
Sep 7, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Natalia A OsnaTerrence M Donohue
Dec 26, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Natalia A OsnaTerrence M Donohue
Oct 30, 1998·Endocrine Reviews·W C DuckworthF G Hamel
Sep 28, 2002·The Journal of Burn Care & Rehabilitation·Cheng-Hui FangPer-Olof Hasselgren
Mar 11, 2004·Journal of Endocrinological Investigation·F G HamelW C Duckworth
Apr 27, 2002·Annals of the New York Academy of Sciences·Alan R HipkissAlbert Ferro
Mar 26, 1998·Biochemical and Biophysical Research Communications·W C DuckworthF G Hamel
May 23, 2001·Archives of Biochemistry and Biophysics·J FawcettW C Duckworth
Dec 19, 2000·Free Radical Biology & Medicine·F S FacchiniR A Stoohs
Jul 10, 2003·Molecular and Cellular Endocrinology·Tomo SaricKresimir Pavelic
Oct 30, 2007·Archives of Biochemistry and Biophysics·Janet FawcettWilliam C Duckworth

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