Insulin-like effect of low-dose leptin on glucose transport in Langendorff rat hearts

Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association
M HaapK Rett

Abstract

In a murine myotube cell line (C 2 C 12 myotubes), leptin at low physiological concentrations (1 ng/ml) has been shown to stimulate glucose transport and glycogen synthesis. The aim of the present study was to test whether an analogous leptin effect on glucose transport is detectable in the heart. We used the isolated Langendorff rat heart preparation with hemodynamic function control. Using polymerase chain reaction (RT-PCR), a 346- and 375-base fragment indicative for the short and long leptin receptor isoform was detected in the rat heart. Glucose transport rates were calculated using equimolar double tracer perfusion with the non-metabolizable glucose analog 3-O-methylglucose (3-O-MG) and the non-transportable tracer mannitol and two-compartimental modeling. 3-O-MG uptake at a perfusate glucose concentration of 11 mM was measured over 15 minutes in control hearts, hearts perfused with insulin (10 mU/ml), leptin (1 ng/ml) or insulin (10 mU/ml) plus leptin (1 ng/ml; n = 8 in each group). The basal 3-O-MG transport rate of 0,7351 +/- 0,051 micro mol/min/g wet weight was increased 4.18 fold with insulin, 2.69 fold with leptin, and 4.2 fold with leptin plus insulin. Simultaneous monitoring of hemodynamic function revealed a mino...Continue Reading

Citations

May 14, 2008·Cardiovascular Research·Morris KarmazynAsad Zeidan
Oct 1, 2011·Clinical and Experimental Pharmacology & Physiology·Ning Hou, Jian-Dong Luo
Nov 2, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Michael E HallDavid E Stec
Jun 15, 2012·European Journal of Nutrition·Javier ModregoAntonio J López-Farré
Aug 19, 2007·Circulation Research·Gary D LopaschukWilliam C Stanley
Dec 2, 2009·Nature Reviews. Cardiology·Gary Sweeney
Mar 13, 2010·Expert Review of Cardiovascular Therapy·Joseph S Dobrin, Djamel Lebeche
May 27, 2020·Current Diabetes Reports·Alexandre A da SilvaJohn E Hall
Nov 19, 2020·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Alexandre A da SilvaJussara M do Carmo

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