Insulin-like growth factor-1 (IGF-1) promotes primordial follicle growth and reduces DNA fragmentation through the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) signalling pathway

Reproduction, Fertility, and Development
Maria É S BezerraM H T Matos

Abstract

We investigated the effects of insulin-like growth factor 1 (IGF-1) on the morphology and follicular activation of ovine preantral follicles cultured in situ and whether the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway is involved in IGF-1 action in the sheep ovary. Ovine ovarian fragments were fixed for histological and terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) analyses (fresh control) or cultured in supplemented alpha-minimum essential medium (α-MEM+; control) or α-MEM+ with IGF-1 (1, 10, 50, 100 or 200ngmL-1) for 7 days. Follicles were classified as normal or atretic, primordial or growing and the oocyte and follicle diameters were measured. DNA fragmentation was evaluated by TUNEL assay. Proliferating cell nuclear antigen (PCNA) immunohistochemistry was performed on the fresh control, α-MEM+ and 100ngmL-1 IGF-1 samples. Inhibition of PI3K activity was performed through pretreatment with the PI3K inhibitor LY294002 and phosphorylated AKT (pAKT) expression was analysed after culture in the absence or presence of LY294002. IGF-1 at 100ngmL-1 increased (P<0.05) follicular activation compared with α-MEM+ and decreased TUNEL-positive cells (P<0.05) compared with other treatments. P...Continue Reading

References

Jan 1, 1991·International Review of Cytology·A N Hirshfield
Nov 1, 1996·Biology of Reproduction·S A WandjiJ E Fortune
Jan 1, 1997·Journal of Reproduction and Fertility·R Braw-Tal, S Yossefi
Apr 25, 2000·Biology of Reproduction·C G GutierrezR Webb
Mar 17, 2004·Cancer Treatment Reviews·Juan Angel Fresno VaraManuel González-Barón
Feb 10, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Courtney A GranvillePhillip A Dennis
Jul 10, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·George B JohnDiego H Castrillon
Jul 8, 2008·Developmental Biology·George B JohnDiego H Castrillon
Sep 17, 2008·Oncogene·C Garcia-Echeverria, W R Sellers
Oct 2, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Eileen A McLaughlin, Skye C McIver
Feb 20, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Maki GotoFumitaka Kikkawa
Oct 13, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Arul Murugan ManiD Claire Wathes
Nov 17, 2009·Trends in Endocrinology and Metabolism : TEM·Pradeep ReddyKui Liu
Aug 21, 2010·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·G Taru SharmaG Sai Kumar
Aug 16, 2011·Molecular and Cellular Endocrinology·Philippe MongetRozenn Dalbies-Tran
Apr 17, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Alexander P SobinoffEileen A McLaughlin
Feb 19, 2013·The International Journal of Developmental Biology·Sandra CecconiFelice Patacchiola
May 23, 2014·Annual Review of Biomedical Engineering·Lonnie D SheaAriella Shikanov
Sep 10, 2014·Endocrine Reviews·Aaron J W HsuehBart C J M Fauser
Oct 28, 2015·Zygote : the Biology of Gametes and Early Embryos·A Y P CavalcanteM H T Matos
Mar 13, 2016·Journal of Ovarian Research·Filiz Tepekoy, Gokhan Akkoyunlu
Jun 28, 2016·Reproduction in Domestic Animals = Zuchthygiene·C R JimenezCaa Torres
Apr 18, 2017·Research in Veterinary Science·F L N AguiarJ R Figueiredo
Jun 9, 2017·Biology of Reproduction·Ricássio S BarberinoMaria Helena T Matos

❮ Previous
Next ❯

Citations

Aug 8, 2020·Endocrine Reviews·Johanne GrosboisIsabelle Demeestere
Jan 6, 2021·Reproductive Biology and Endocrinology : RB&E·Jiayi SunHaibin Kuang

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.