Insulin-like growth factor 3 regulates expression of genes encoding steroidogenic enzymes and key transcription factors in the Nile tilapia gonad

Biology of Reproduction
Minghui LiD S Wang

Abstract

Insulin-like growth factors (Igfs) are implicated in a wide variety of physiological roles in teleost gonadal development and reproduction. In the present study, igf3 mRNA expression in the tilapia ovary was found to be higher than in the testis from 5 to 40 days after hatching (dah) but was lower than that in testis from 50 to 70 dah. Consistently, Igf3 protein signal was detected in the somatic cells of XX and XY gonads from 10 dah until adulthood by immunohistochemistry, using a specific Igf3 polyclonal antibody. Incubation of ovarian and testicular cells in primary culture with recombinant Igf3 significantly increased nr5a1, foxl2, dmrt1, cyp19a1a, cyp11a1, cyp11b2, hsd3b2 , and cyp17a1 expression in a time- and dose-dependent manner. Promoter analysis using luciferase assays in HEK293 cells revealed that igf3 promoter activity was directly activated by Nr5a1 (Sf1) and further enhanced by Foxl2, Nr0b1a (Dax1), and Nr0b1b (Dax2) but repressed by Dmrt1 and estrogen receptor (Esr1, Esr2a, or Esr2b) along with 17beta-estradiol treatment. In addition, igf3 promoter activity was increased slightly by forskolin treatment alone but synergistically up-regulated by transfection with nr5a1. These in vitro results correlated well with ...Continue Reading

References

Nov 1, 1992·Journal of Pediatric Surgery·I H KrasnaL Eierman
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T MiuraY Nagahama
Feb 1, 1994·Journal of Molecular Endocrinology·S J DuguayW W Dickhoff
Jun 1, 1997·Endocrine Reviews·K L Parker, B P Schimmer
Sep 13, 2002·General and Comparative Endocrinology·Kazuyuki ShibataMasahisa Nakamura
Jan 21, 2003·Biology of Reproduction·Craig A SmithAndrew H Sinclair
Jun 23, 2004·Biochemical and Biophysical Research Communications·Deshou WangYoshitaka Nagahama
Mar 31, 2005·International Review of Cytology·Antony W WoodHoward A Bern
May 3, 2005·General and Comparative Endocrinology·Manfred ReineckeJoaquim Gutiérrez
Aug 24, 2005·General and Comparative Endocrinology·Ramji Kumar BhandariYoshitaka Nagahama
Mar 15, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Dilip MukherjeeS P Bhattacharyya
Aug 12, 2006·Journal of Molecular Endocrinology·Amy L FilbyCharles R Tyler
Feb 27, 2007·General and Comparative Endocrinology·Gregory M WeberCraig V Sullivan
Sep 15, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Shunsuke MoriyamaHiroshi Kawauchi
Jan 2, 2008·Biochemical and Biophysical Research Communications·De-Shou WangChristopher H K Cheng
Mar 18, 2009·General and Comparative Endocrinology·Yann GuiguenChing-Fong Chang
May 4, 2010·General and Comparative Endocrinology·Sharon N Nelson, Glen Van Der Kraak

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Citations

Oct 10, 2012·Journal of Molecular Endocrinology·Elisabeth SambroniFlorence Le Gac
Jul 25, 2014·General and Comparative Endocrinology·Jean-François BaroillerManfred Reinecke
Jul 16, 2014·Journal of Molecular Endocrinology·François ChauvignéJoan Cerdà
Jan 14, 2016·The Journal of Endocrinology·Lili ChenDeshou Wang
Apr 23, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Huirong YangHaoran Lin
Jul 25, 2019·Molecular Reproduction and Development·Xianbo ZhangDeshou Wang
Jul 25, 2015·Endocrinology·Rafael Henrique NóbregaJan Bogerd
Jun 27, 2018·Biology of Reproduction·Jianzhen LiChristopher H K Cheng
Aug 23, 2020·International Journal of Molecular Sciences·Mimi JieDeshou Wang
Aug 15, 2020·Frontiers in Endocrinology·Michelle ThönnesFrank Pfennig
Dec 5, 2020·Biology of Reproduction·Yuxin XieChristopher H K Cheng
Mar 12, 2021·Biology of Reproduction·Jianzhen LiChristopher H K Cheng
Jul 6, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Hongjuan ShiGuangli Li

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Methods Mentioned

BETA
PCR
ELISA
X-ray
transfection
electrophoresis
transfections

Software Mentioned

GraphPad Prism

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