Insulin-like growth factor and epidermal growth factor independence in human mammary carcinoma cells with c-erbB-2 gene amplification and progressively elevated levels of tyrosine-phosphorylated p185erbB-2

Molecular Carcinogenesis
T G RamStephen P Ethier

Abstract

Growth factor-independent proliferation is an essential aspect of the transformation process. To study the influence of c-erbB-2 overexpression on the autonomous growth of human mammary cancer cells, we used a series of non-neoplastic and neoplastic human mammary epithelial cell lines isolated from a patient with intraductal and invasive ductal carcinoma of the breast. The non-neoplastic cell line, H16N-2, which expresses a normal level (single gene copy) of c-erbB-2, was used for comparison with the neoplastic cell lines. Both the metastatic tumor cell lines, 21MT-1 and 21 MT-2, showed equivalent amplification of the c-erbB-2 gene; however, 21MT-1 cells showed a higher level of c-erbB-2 overexpression. Therefore, the H16N-2, 21MT-2, and 21MT-1 cell series forms a distinct gradient of progressively increasing c-erbB-2 gene expression. Furthermore, the overexpression of c-erbB-2 in the 21MT cell lines was concordant with increases in the constitutive tyrosine kinase activity of p185erb-2 measured in the absence of exogenous growth factors in culture. Normal mammary epithelial cells require both insulin-like growth factor (IGF)-l (or supraphysiological concentrations of insulin) and epidermal growth factor (EGF) to proliferate un...Continue Reading

References

May 22, 1992·Science·W E HolmesG D Lewis
Nov 22, 1991·Science·S A Aaronson
Jan 11, 1991·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·T M WilliamsH C Maguire
Jun 1, 1990·Molecular and Cellular Biology·E Di MarcoP P Di Fiore
Apr 20, 1990·Cell·A Ullrich, J Schlessinger
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M H KrausS A Aaronson
Jul 10, 1987·Science·P P Di FioreS A Aaronson
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R M HudziakA Ullrich
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·V Band, R Sager
Nov 30, 1987·Biochemical and Biophysical Research Communications·Q T RohlikS Jacobs
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S L HammondM R Stampfer
May 1, 1980·In Vitro·M StampferA J Hackett
Jun 1, 1994·Molecular and Cellular Biology·S P SoltoffL C Cantley
Dec 2, 1993·Nature·G D PlowmanS Buckley
Jul 25, 1994·FEBS Letters·Y A KitaM Nicolson
May 8, 1993·International Journal of Cancer. Journal International Du Cancer·M al-KasspoolesD G Beer
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G D PlowmanM Shoyab
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M H KrausS A Aaronson

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Citations

Jul 6, 2007·Breast Cancer Research and Treatment·Ashok SubramanianKefah Mokbel
Feb 13, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·G KrähnR U Peter
May 2, 2009·The Journal of Biological Chemistry·Wei-Qin ZhaoWilliam L Klein
Dec 26, 2001·Journal of the National Cancer Institute·J Albanell, J Baselga
Aug 2, 2005·American Journal of Respiratory Cell and Molecular Biology·Gunamani SithanandamLucy M Anderson
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·G NocentiniC Riccardi
Feb 11, 2005·International Journal of Cancer. Journal International Du Cancer·Edward Htun van der HorstAxel Ullrich
Oct 7, 2005·Neoplasia : an International Journal for Oncology Research·Kathleen M Woods IgnatoskiStephen P Ethier
Nov 11, 2006·International Journal of Cancer. Journal International Du Cancer·Armin KolbHelmut Friess
Dec 22, 1999·British Journal of Cancer·F ForozanS P Ethier
Jul 1, 2005·Molecular and Cellular Biology·Juinn-Lin LiuW K Alfred Yung
Dec 21, 2006·Cancer Research·Zeng Quan YangStephen P Ethier
Aug 10, 2019·Experimental Cell Research·Maddaly RaviAastha Joshipura

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