Insulin-like growth factor-binding protein-5 inhibits growth and induces differentiation of mouse osteosarcoma cells

Biochemical and Biophysical Research Communications
M R SchneiderHarald Lahm

Abstract

The precise role of insulin-like growth factor-binding protein-5 (IGFBP-5) in regulating the growth of tumor cells, especially of bone-derived malignant cells, is not well understood. We have investigated the biological activity of IGFBP-5 by transfecting OS/50-K8 mouse osteosarcoma cells with an expression vector containing the osteocalcin promoter and the complete mouse IGFBP-5 cDNA (OC-IGFBP-5). Overexpression of IGFBP-5 mRNA and secretion of increased amounts of bioactive protein in conditioned media were demonstrated in different clones. For the analysis of cell proliferation, three clones exhibiting high levels of IGFBP-5 expression were selected and compared to a mock clone and to nontransfected parental cells. IGFBP-5-secreting clones displayed reduced proliferation under both anchorage-dependent and -independent conditions (P < 0.05). The increase in proliferation observed in IGFBP-5-secreting clones after addition of exogenous IGF was significantly lower than that observed in mock-transfected or parental cells. A similar result was obtained with long[R3]IGF-I which has a low affinity for all IGFBPs, suggesting that the inhibitory effect of IGFBP-5 is only partially IGF-dependent. OC-IGFBP-5-transfected clones expresse...Continue Reading

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Citations

Aug 20, 2008·Breast Cancer Research : BCR·Mustafa AkkiprikWei Zhang
Nov 26, 2009·Experimental and Molecular Pathology·Marlon R SchneiderThomas Klonisch
Aug 18, 2009·Molecular and Cellular Endocrinology·Marjeta GrzechMarlon R Schneider
Feb 24, 2007·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Aditi Mukherjee, Peter Rotwein
Jun 5, 2004·Genomics·Celine PompeiaBechara Kachar
Oct 15, 2011·Genesis : the Journal of Genetics and Development·Maik DahlhoffMarlon R Schneider
May 1, 2012·Asian-Australasian Journal of Animal Sciences·X J WangD J Liu
Oct 11, 2002·The Journal of Pathology·Shu-Chun LinKuo-Wei Chang
Oct 6, 2005·American Journal of Physiology. Endocrinology and Metabolism·Daniela KiepeBurkhard Tönshoff
Jul 23, 2005·World Journal of Gastroenterology : WJG·Xue-Qin GaoChang-Zheng Song
Mar 21, 2020·Frontiers in Endocrinology·Cumming Duan, John B Allard
Mar 11, 2006·The Biochemical Journal·James BeattieDavid J Flint
Jun 5, 2003·The Journal of Biological Chemistry·Alison J ButtRobert C Baxter

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