Insulin-like growth factor-I increases bone sialoprotein (BSP) expression through fibroblast growth factor-2 response element and homeodomain protein-binding site in the proximal promoter of the BSP gene

Journal of Cellular Physiology
Youhei NakayamaYorimasa Ogata

Abstract

Insulin-like growth factor-I (IGF-I) promotes bone formation by stimulating proliferation and differentiation of osteoblasts. Bone sialoprotein (BSP), is thought to function in the initial mineralization of bone, is selectively expressed by differentiated osteoblast. To determine the molecular mechanism of IGF-I regulation of osteogenesis, we analyzed the effects of IGF-I on the expression of BSP in osteoblast-like Saos2 and in rat stromal bone marrow (RBMC-D8) cells. IGF-I (50 ng/ml) increased BSP mRNA levels at 12 h in Saos2 cells. In RBMC-D8 cells, IGF-I increased BSP mRNA levels at 3 h. From transient transfection assays, a twofold increase in transcription by IGF-I was observed at 12 h in pLUC3 construct that included the promoter sequence from -116 to +60. Effect of IGF-I was abrogated by 2-bp mutations in either the FGF2 response element (FRE) or homeodomain protein-binding site (HOX). Gel shift analyses showed that IGF-I increased binding of nuclear proteins to the FRE and HOX elements. Notably, the HOX-protein complex was supershifted by Smad1 antibody, while the FRE-protein complex was shifted by Smad1 and Cbfa1 antibodies. Dlx2 and Dlx5 antibodies disrupted the formation of the FRE- and HOX-protein complexes. The IGF...Continue Reading

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Citations

Feb 23, 2008·Nature Reviews. Cancer·Akeila BellahcèneNeal S Fedarko
Aug 24, 2013·PloS One·Shigeo KawadaNaokata Ishii
Jul 1, 2010·Journal of Oral Science·Xinyue LiYorimasa Ogata
Jul 1, 2010·Journal of Oral Science·Li YangYorimasa Ogata
Apr 7, 2011·Journal of Oral Science·Yoko SasakiYorimasa Ogata
May 10, 2008·Archives of Physiology and Biochemistry·Francesco FrascaRiccardo Vigneri
Dec 26, 2012·Gene·Liming ZhouYorimasa Ogata
Dec 5, 2008·Journal of Periodontal Research·Y NakayamaY Ogata
Feb 28, 2008·Journal of Periodontal Research·Y Ogata
Jun 22, 2010·Journal of Cellular Biochemistry·Li YangYorimasa Ogata
Apr 12, 2012·Journal of Cellular Biochemistry·Xinyue LiYorimasa Ogata
Jun 22, 2010·Journal of Cellular Biochemistry·Xinyue LiYorimasa Ogata
Apr 18, 2014·Journal of Oral Science·Hiroyoshi Matsumura, Yorimasa Ogata
Mar 15, 2013·Journal of Oral Science·Liming Zhou, Yorimasa Ogata
Sep 18, 2013·Journal of Oral Science·Hideki TakaiYorimasa Ogata
Mar 19, 2014·Journal of Bone and Mineral Metabolism·Hiroyoshi MatsumuraYorimasa Ogata
Jan 24, 2007·Journal of Cellular Biochemistry·Dong-Soon KimYorimasa Ogata

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