Insulin-like growth factor I is an independent coregulatory modulator of natural killer (NK) cell activity

Endocrinology
C J AuernhammerC J Strasburger

Abstract

We aimed to investigate the natural killer (NK) cell activity in hGH-deficient adults and to analyze the effect of insulin-like growth factor (IGF)-I in vivo and in vitro on NK cell activity. NK cell activity was measured in a 4-h nonisotopic assay with europium-labeled and cryopreserved K-562 cells. NK-cell numbers were measured after incubation with murine monoclonal CD3 and CD16 antibodies by flow cytometry analysis. In a cross-sectional study, the basal and interferon-beta (IFN-beta) stimulated (1000 IU/ml) NK cell activity of 15 hGH-deficient patients and 15 age- and sex-matched controls was measured. The percentages and absolute numbers of CD3-/16+ NK-cells were not significantly different in the patient vs. control group. The basal and IFN-beta stimulated NK cell activity however was significantly decreased in the patient vs. control group at all effector/target (E/T) cell ratios from 12.5-100 (e.g. 17 +/- 3 vs. 28 +/- 3% lysis without IFN-beta, P < 0.05, and 42 +/- 4 vs. 57 +/- 4% lysis with IFN-beta, P < 0.05; both at E/T 50). IGF-I levels of patients and controls showed a significant positive correlation with NK cell activity (r = 0.37; P < 0.05). In an IGF-I in vitro study (IGF-I in vitro 250-1250 microg/L), the basa...Continue Reading

Citations

Dec 24, 1997·Acta Paediatrica. Supplement·M BidlingmaierC J Strasburger
Jun 13, 2000·JPEN. Journal of Parenteral and Enteral Nutrition·J TackeK W Jauch
Jan 31, 2019·Clinical and Experimental Immunology·M R HoltR M Thomson
Sep 22, 2007·Critical Care Medicine·Virginie MaximeDjillali Annane
Nov 23, 2000·Cancer Control : Journal of the Moffitt Cancer Center·E A Burns, E A Leventhal

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