Insulin-like growth factor-I regulates aromatase activity in human granulosa and granulosa luteal cells

The Journal of Clinical Endocrinology and Metabolism
G F EricksonD A Magoffin

Abstract

The purpose of this research was to test the hypothesis that insulin-like growth factor-I (IGF-I) regulates estradiol (E2) synthesis in human granulosa and granulosa luteal cells. Cells from individual follicles from spontaneous and human menopausal gonadotropin/CG-stimulated cycles were cultured in serum-free medium containing androstenedione, IGF-I, FSH, and/or CG. At 2, 4, and 6 days, E2 in the medium was measured by RIA. In the granulosa experiments, control cells produced basal levels of E2 at 2 days, and the levels increased with increasing follicle size. Treatment with FSH stimulated E2 production (on the average, 5-fold), and the effect was dose dependent (ED50 = 5 ng/mL or 16 mIU/mL). Incubation with IGF-I alone caused increases in E2 production comparable to those caused by FSH, and the IGF-I effect was dose dependent (ED50 = 8 ng/mL). In most cases, coincubation with FSH and IGF-I augmented E2 levels more than either hormone alone, and at 4 and 6 days the interaction was synergistic. The data from dose-response experiments suggested that the basis of the synergy between FSH and IGF-I was a marked potentiation by either hormone (approximately 10-fold) in the potency of the complementary hormone to stimulate E2 product...Continue Reading

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